Grebinyk Anna, Grebinyk Sergii, Prylutska Svitlana, Ritter Uwe, Matyshevska Olga, Dandekar Thomas, Frohme Marcus
Division Molecular Biotechnology and Functional Genomics, Technical University of Applied Sciences Wildau, Hochschulring 1, 15745 Wildau, Germany.
Dept. of Bioinformatics, Biocenter, University of Würzburg, Am Hubland, 97074 Würzburg, Germany.
Data Brief. 2018 Jul 10;19:2047-2052. doi: 10.1016/j.dib.2018.06.089. eCollection 2018 Aug.
The presented dataset describes the quantification of carbon nanoparticle C fullerene accumulated in mitochondria of human leukemic cells treated with nanostructure. Firstly, the high performance liquid chromatography-electro spray ionization-mass spectrometry (HPLC-ESI-MS) method was developed for quantitative analysis of pristine C fullerene. Then, human leukemic cells were incubated with C fullerene, homogenized and subjected to the differential centrifugation to retrieve mitochondrial fraction. The C fullerene content was quantified by HPLC-ESI-MS in extracts of cellular fractions. This data article refers to the research article "C Fullerene Accumulation in Human Leukemic Cells and Perspectives of LED-mediated Photodynamic Therapy" by Grebinyk et al. [1].
所呈现的数据集描述了用纳米结构处理的人类白血病细胞线粒体中积累的碳纳米颗粒C富勒烯的定量情况。首先，开发了高效液相色谱-电喷雾电离-质谱（HPLC-ESI-MS）方法用于原始C富勒烯的定量分析。然后，将人类白血病细胞与C富勒烯一起孵育，匀浆并进行差速离心以获取线粒体部分。通过HPLC-ESI-MS对细胞部分提取物中的C富勒烯含量进行定量。本数据文章参考了Grebinyk等人的研究文章《C富勒烯在人类白血病细胞中的积累及LED介导的光动力疗法的前景》[1]。
