Division Molecular Biotechnology and Functional Genomics, Technical University of Applied Sciences Wildau, Hochschulring 1, 15745 Wildau, Germany.
Department of Chemistry, Taras Shevchenko National University of Kyiv, Volodymyrska 64, 01601 Kyiv, Ukraine.
Int J Mol Sci. 2023 Jan 5;24(2):1020. doi: 10.3390/ijms24021020.
The acoustic pressure waves of ultrasound (US) not only penetrate biological tissues deeper than light, but they also generate light emission, termed sonoluminescence. This promoted the idea of its use as an alternative energy source for photosensitizer excitation. Pristine C fullerene (C), an excellent photosensitizer, was explored in the frame of cancer sonodynamic therapy (SDT). For that purpose, we analyzed C effects on human cervix carcinoma HeLa cells in combination with a low-intensity US treatment. The time-dependent accumulation of C in HeLa cells reached its maximum at 24 h (800 ± 66 ng/10 cells). Half of extranuclear C is localized within mitochondria. The efficiency of the C nanostructure's sonoexcitation with 1 MHz US was tested with cell-based assays. A significant proapoptotic sonotoxic effect of C was found for HeLa cells. C's ability to induce apoptosis of carcinoma cells after sonoexcitation with US provides a promising novel approach for cancer treatment.
超声波（US）的声压波不仅比光穿透生物组织更深，而且还会产生光发射，称为声致发光。这促进了将其用作光敏剂激发的替代能源的想法。原始 C 富勒烯（C）是一种出色的光敏剂，在癌症声动力学疗法（SDT）的框架内进行了探索。为此，我们分析了 C 对人宫颈癌细胞系 HeLa 的影响，结合低强度 US 处理。C 在 HeLa 细胞中的时间依赖性积累在 24 小时时达到最大值（800 ± 66 ng/10 个细胞）。一半的核外 C 定位于线粒体中。用基于细胞的测定法测试了 1 MHz US 对 C 纳米结构的声激发效率。发现 C 对 HeLa 细胞具有显著的促凋亡声毒性作用。C 在超声声激发后诱导癌细胞凋亡的能力为癌症治疗提供了一种有前途的新方法。
