Department of Surgery for Vascular Thyroid and Hernia, Xuzhou Central Hospital, Xuzhou, China.
Eur Rev Med Pharmacol Sci. 2018 Sep;22(17):5562-5568. doi: 10.26355/eurrev_201809_15819.
To investigate the significance and possible mechanism of miR-791 in the pathogenesis of papillary thyroid carcinoma (PTC).
The expression of miR-791 in 80 cases of thyroid carcinoma tissues and 80 cases of paracancerous tissues was detected by quantitative Real-time-polymerase chain reaction (qRT-PCR). After miR-791 mimics were transfected into thyroid cancer cells by liposome method, the cell proliferation was detected by Cell Counting Kit-8 (CCK-8) and 5-ethynyl-2'-deoxyuridine (EDU), respectively. Cell cycle was detected by flow cytometry.
The expression of miR-791 in thyroid cancer tissue was significantly lower than that of normal thyroid. The mir-719 expression is positively correlated with the prognosis of thyroid carcinoma. After transfection of miR-791 mimics, the proliferation ability of TPC-1 and HTH83 cells was weakened, and the cell cycle was blocked in the G0/G1 phase. Further study on the underlying mechanism found that after overexpression of miR-791, the expressions of Cyclin D1, CKD6 and CDK4 decreased significantly, while the expression of cyclin inhibitor P21 increased significantly.
MiR-791 is lowly expressed in thyroid cancer. MiR-791 may inhibit thyroid cancer cell proliferation by blocking thyroid cancer cells in G0/G1 phase, thus participating in the impediment of thyroid cancer development.
探讨 miR-791 在甲状腺癌（PTC）发病机制中的意义和可能机制。
采用实时荧光定量聚合酶链反应（qRT-PCR）检测 80 例甲状腺癌组织和 80 例癌旁组织中 miR-791 的表达。采用脂质体法将 miR-791 模拟物转染入甲状腺癌细胞后，分别用细胞计数试剂盒（CCK-8）和 5-乙炔基-2'-脱氧尿苷（EDU）检测细胞增殖。用流式细胞术检测细胞周期。
甲状腺癌组织中 miR-791 的表达明显低于正常甲状腺。mir-719 的表达与甲状腺癌的预后呈正相关。转染 miR-791 模拟物后，TPC-1 和 HTH83 细胞的增殖能力减弱，细胞周期被阻滞在 G0/G1 期。进一步的机制研究发现，过表达 miR-791 后，Cyclin D1、CKD6 和 CDK4 的表达明显下降，而细胞周期蛋白抑制剂 P21 的表达明显增加。
miR-791 在甲状腺癌中低表达。miR-791 可能通过阻滞甲状腺癌细胞在 G0/G1 期来抑制甲状腺癌细胞增殖，从而参与甲状腺癌的发生发展。
