长链非编码 RNA GAS8-AS1 通过 miR-135b-5p/CCND2 轴抑制甲状腺乳头状癌细胞生长。
LncRNA GAS8-AS1 suppresses papillary thyroid carcinoma cell growth through the miR-135b-5p/CCND2 axis.
机构信息
General Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China.
General Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China
出版信息
Biosci Rep. 2019 Jan 11;39(1). doi: 10.1042/BSR20181440. Print 2019 Jan 31.
The aim of the present study was to investigate the potential role of GAS8 antisense RNA 1 (GAS8-AS1) in papillary thyroid carcinoma (PTC). PcDNA3.1-GAS8-AS1 and si-GAS8-AS1, miR-135b-5p mimic and si-CCND2 were transfected into PTC cells. Cell proliferation was evaluated by Cell Counting Kit-8 (CCK-8). QRT-PCR was used to determine expressions of GAS8-AS1, miR-135b-5p, and CCND2, and Western blot were detected protein level of CCND2. The miRNA target gene prediction site TargetScan was used to predict potential targets of GAS8-AS1 and miR-135b-5p. Cell cycle progression was analyzed by flow cytometry. We found that GAS8-AS1 was down-regulated in PTC cell lines and inhibited proliferation and cycle of PTC cell. GAS8-AS1 directly targets miR-135b-5p, and GAS8-AS1 could regulate a downstream target of miR-135b-5p, Cyclin G2 (CCNG2), in an miR-135b-5p-mediated manner. In addition, we also proved that overexpressed GAS8-AS1 inhibited tumor formation GAS8-AS1 suppresses PTC cell growth through the miR-135b-5p/CCND2 axis.
本研究旨在探讨 GAS8 反义 RNA 1（GAS8-AS1）在甲状腺乳头状癌（PTC）中的潜在作用。将 PcDNA3.1-GAS8-AS1 和 si-GAS8-AS1、miR-135b-5p 模拟物和 si-CCND2 转染到 PTC 细胞中。通过细胞计数试剂盒-8（CCK-8）评估细胞增殖。使用 QRT-PCR 测定 GAS8-AS1、miR-135b-5p 和 CCND2 的表达，并通过 Western blot 检测 CCND2 的蛋白水平。miRNA 靶基因预测网站 TargetScan 用于预测 GAS8-AS1 和 miR-135b-5p 的潜在靶基因。通过流式细胞术分析细胞周期进程。我们发现 GAS8-AS1 在 PTC 细胞系中下调，并抑制 PTC 细胞的增殖和周期。GAS8-AS1 直接靶向 miR-135b-5p，并且 GAS8-AS1 可以以 miR-135b-5p 介导的方式调节 miR-135b-5p 的下游靶基因，细胞周期蛋白 G2（CCNG2）。此外，我们还证明过表达 GAS8-AS1 抑制肿瘤形成。GAS8-AS1 通过 miR-135b-5p/CCND2 轴抑制 PTC 细胞生长。
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