School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, Singapore, 637459, Singapore.
NTU-Northwestern Institute for Nanomedicine, Nanyang Technological University, 50 Nanyang Avenue, Singapore, 639798, Singapore.
Chembiochem. 2019 Feb 15;20(4):437-450. doi: 10.1002/cbic.201800430. Epub 2018 Nov 20.
Timely monitoring and assessment of human health plays a crucial role in maintaining the wellbeing of our advancing society. In addition to medical tools and devices, suitable probe agents are crucial to assist such monitoring, either in passive or active ways (i.e., sensors) through inducible signals. In this review we highlight recent developments in activatable optical sensors based on nucleic acids. Sensing mechanisms and bio-applications of these nucleic acid sensors in ex vivo assays, intracellular or in vivo settings are described. In addition, we discuss the limitations of these sensors and how nanotechnology can complement/enhance sensor properties to promote translation into clinical applications.
及时监测和评估人类健康对于维护我们不断发展的社会的健康至关重要。除了医疗工具和设备外，合适的探针试剂对于通过诱导信号以被动或主动方式（即传感器）进行这种监测也至关重要。在这篇综述中，我们重点介绍了基于核酸的新型可激活光学传感器的最新进展。描述了这些核酸传感器的传感机制和在体外分析、细胞内或体内环境中的生物应用。此外，我们还讨论了这些传感器的局限性以及纳米技术如何补充/增强传感器性能以促进向临床应用的转化。
