Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, United States; DOE Center for Advanced Bioenergy and Bioproducts Innovation, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, United States.
Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, United States; DOE Center for Advanced Bioenergy and Bioproducts Innovation, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, United States.
Curr Opin Chem Biol. 2020 Aug;57:95-104. doi: 10.1016/j.cbpa.2020.05.003. Epub 2020 Jul 8.
Imaging of species in living organisms with high spatiotemporal resolution is essential for understanding biological processes. While functional nucleic acids (FNAs), such as catalytic nucleic acids and aptamers, have emerged as effective sensors for a wide range of molecules, photocaged control of these FNAs has played a key role in translating them into bioimaging agents with high spatiotemporal control. In this review, we summarize methods and results of photocaged FNAs based on photolabile modifications, photoisomerization, and photothermal activation. Future directions, including strategies to improve the performance of these photocaged FNAs, are also described.
用高时空分辨率对活体生物中的物种进行成像，对于理解生物过程至关重要。虽然功能核酸（FNAs），如催化核酸和适体，已成为广泛分子的有效传感器，但这些 FNAs 的光笼控制在将其转化为具有高时空控制的生物成像剂方面发挥了关键作用。在这篇综述中，我们总结了基于光不稳定修饰、光致异构化和光热激活的光笼 FNAs 的方法和结果。还描述了未来的方向，包括提高这些光笼 FNAs 性能的策略。
