Jones I M, Reay P A, Philpott K L
EMBO J. 1986 Sep;5(9):2371-6. doi: 10.1002/j.1460-2075.1986.tb04506.x.
In order to re-examine the sub-cellular location of the three influenza A/NT/60/68 polymerase proteins PB1, PB2 and PA in infected cells, specific antisera for each polymerase component have been prepared by immunizing rabbits with polymerase-beta-galactosidase fusion proteins synthesized in Escherichia coli. We show that polymerase PB1, PB2, and PA are predominantly associated with the nucleus of influenza-infected MDCK cells by immunocytochemical techniques. In the case of polymerase PB2 we investigate the possibility that nuclear accumulation is an intrinsic property of the PB2 protein. Using a vaccinia-PB2 recombinant virus, we show that PB2 accumulates intra-nuclearly in monkey CV-1 cells in the absence of any other influenza protein, suggesting it contains an intrinsic nuclear signal.
为了重新研究甲型流感病毒A/NT/60/68的三种聚合酶蛋白PB1、PB2和PA在感染细胞中的亚细胞定位，通过用在大肠杆菌中合成的聚合酶-β-半乳糖苷酶融合蛋白免疫兔子，制备了针对每种聚合酶组分的特异性抗血清。我们通过免疫细胞化学技术表明，聚合酶PB1、PB2和PA主要与流感感染的MDCK细胞核相关。对于聚合酶PB2，我们研究了核积累是PB2蛋白固有特性的可能性。使用痘苗-PB2重组病毒，我们表明在没有任何其他流感蛋白的情况下，PB2在猴CV-1细胞核内积累，这表明它含有一个固有核信号。
