Le Anh V, Huynh Tien T, Parks Sophie E, Nguyen Minh H, Roach Paul D
School of Environmental and Life Sciences, University of Newcastle, Ourimbah, NSW 2258, Australia.
Faculty of Bio-Food Technology and Environment, University of Technology (HUTECH), Ho Chi Minh City 700000, Vietnam.
Medicines (Basel). 2018 Sep 18;5(3):104. doi: 10.3390/medicines5030104.
Gac ( Spreng) seeds have long been used in traditional medicine as a remedy for numerous conditions due to a range of bioactive compounds. This study investigated the solvent extraction of compounds that could be responsible for antioxidant activity and anticancer potential. Defatted Gac seed kernel powder was extracted with different solvents: 100% water, 50% methanol:water, 70% ethanol:water, water saturated butanol, 100% methanol, and 100% ethanol. Trypsin inhibitors, saponins, phenolics, and antioxidant activity using the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and the ferric reducing antioxidant power (FRAP) assays; and anticancer potential against two melanoma cancer cell lines (MM418C1 and D24) were analysed to determine the best extraction solvents. Water was best for extracting trypsin inhibitors (581.4 ± 18.5 mg trypsin/mg) and reducing the viability of MM418C1 and D24 melanoma cells (75.5 ± 1.3 and 66.9 ± 2.2%, respectively); the anticancer potential against the MM418C1 cells was highly correlated with trypsin inhibitors (r = 0.92, < 0.05), but there was no correlation between anticancer potential and antioxidant activity. The water saturated butanol had the highest saponins (71.8 ± 4.31 mg aescin equivalents/g), phenolic compounds (20.4 ± 0.86 mg gallic acid equivalents/g), and antioxidant activity, but these measures were not related to anticancer potential. Water yielded a Gac seed extract, rich in trypsin inhibitors, which had high anticancer potential against two melanoma cell lines.
由于富含多种生物活性化合物，罗汉果（Spreng）种子长期以来一直被用于传统医学，治疗多种疾病。本研究调查了罗汉果种子中可能具有抗氧化活性和抗癌潜力的化合物的溶剂萃取情况。用不同溶剂对脱脂罗汉果种子仁粉进行萃取：100%水、50%甲醇:水、70%乙醇:水、水饱和丁醇、100%甲醇和100%乙醇。采用2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）二铵盐（ABTS）、2,2-二苯基-1-苦基肼（DPPH）和铁还原抗氧化能力（FRAP）测定法分析胰蛋白酶抑制剂、皂苷、酚类物质和抗氧化活性；并分析了对两种黑色素瘤癌细胞系（MM418C1和D24）的抗癌潜力，以确定最佳萃取溶剂。水最适合萃取胰蛋白酶抑制剂（581.4±18.5毫克胰蛋白酶/毫克），并降低MM418C1和D24黑色素瘤细胞的活力（分别为75.5±1.3%和66.9±2.2%）；对MM418C1细胞的抗癌潜力与胰蛋白酶抑制剂高度相关（r = 0.92，P<0.05），但抗癌潜力与抗氧化活性之间没有相关性。水饱和丁醇的皂苷含量最高（71.8±4.31毫克七叶皂苷当量/克）、酚类化合物含量最高（20.4±0.86毫克没食子酸当量/克），抗氧化活性也最高，但这些指标与抗癌潜力无关。水萃取得到的罗汉果种子提取物富含胰蛋白酶抑制剂，对两种黑色素瘤细胞系具有较高的抗癌潜力。
