（罗汉果）种子提取物诱导黑色素瘤细胞凋亡和坏死。

(Gấc) Seed Extracts Induce Apoptosis and Necrosis in Melanoma Cells.

作者信息

Nguyen Dao, Holien Jessica, Dekiwadia Chaitali, Thrimawithana Thilini, Piva Terrence, Huynh Tien

机构信息

School of Science, RMIT University, P.O. Box 71, Bundoora 3083, Australia.

Faculty of Agriculture and Forestry, Tay Nguyen University, 567 Le Duan Street, Buon Ma Thuot City 63000, Vietnam.

出版信息

Pharmaceuticals (Basel). 2023 Jan 9;16(1):100. doi: 10.3390/ph16010100.

DOI:10.3390/ph16010100

PMID:36678596

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9864523/

Abstract

is a herbal medicine used throughout Asia and this study investigated the antimelanoma potentials and molecular mechanisms of seed with emphasis on extraction to optimise bioactivity. Overall, the aqueous extract was superior, with a wider diversity and higher concentration of proteins and peptides that was more cytotoxic to the melanoma cells than other extraction solvents. The IC50 of the aqueous extract on melanoma cells were similar to treatment with current anticancer drugs, vemurafenib and cisplatin. This cytotoxicity was cancer-specific with lower cytotoxic effects on HaCaT epidermal keratinocytes. Cytotoxicity correlated with MAPK signalling pathways leading to apoptosis and necrosis induced by triggering tumour necrosis factor receptor-1 (TNFR1), reducing the expression of nuclear factor kappa B (NF-kB), and suppression of BRAF/MEK. This efficacy of seed extracts on melanoma cells provides a platform for future clinical trials as potent adjunctive therapy for metastatic melanoma.

摘要

是一种在亚洲各地都有使用的草药，本研究调查了其种子的抗黑色素瘤潜力和分子机制，重点在于提取以优化生物活性。总体而言，水提取物更具优势，其蛋白质和肽的种类更多、浓度更高，对黑色素瘤细胞的细胞毒性比其他提取溶剂更强。水提取物对黑色素瘤细胞的半数抑制浓度（IC50）与当前抗癌药物维莫非尼和顺铂治疗相似。这种细胞毒性具有癌症特异性，对HaCaT表皮角质形成细胞的细胞毒性较低。细胞毒性与丝裂原活化蛋白激酶（MAPK）信号通路相关，该通路通过触发肿瘤坏死因子受体-1（TNFR1）导致凋亡和坏死，降低核因子κB（NF-κB）的表达，并抑制BRAF/MEK。种子提取物对黑色素瘤细胞的这种功效为未来作为转移性黑色素瘤有效辅助治疗的临床试验提供了一个平台。