从腐生拟青霉中分离得到的一种新型多烯类抗生素——佩斯汀。

Pestiocandin, a new papulacandin class antibiotic isolated from Pestalotiopsis humus.

机构信息

Graduate School of Infection Control Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo, 108-8641, Japan.

Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo, 108-8641, Japan.

出版信息

J Antibiot (Tokyo). 2018 Nov;71(12):1031-1035. doi: 10.1038/s41429-018-0102-7. Epub 2018 Sep 19.

DOI:10.1038/s41429-018-0102-7

PMID:30232378

Abstract

Secondary metabolites of microorganisms have proven to be an excellent source of drugs. We isolated a new antibiotic, named pestiocandin (1), from a culture broth of a filamentous fungus, Pestalotiopsis humus FKI-7473, using a multidrug-sensitive budding yeast, S. cerevisiae 12geneΔ0HSR-iERG6. The structure of 1 was elucidated by various NMR studies. All geometric isomerisms of 1 were shown to be the E-form and two pyranose units of 1 were found to be glucose and galactose types. Compound 1 showed weak growth inhibition against Gram-positive and Gram-negative bacteria, yeasts and a filamentous fungus. It displayed more potent growth inhibition against multidrug-sensitive yeasts than wild-type yeasts.

摘要

微生物的次生代谢产物已被证明是药物的极好来源。我们从丝状真菌 Pestalotiopsis humus FKI-7473 的发酵液中分离到一种新的抗生素，命名为 pestiocandin（1），使用多药敏感出芽酵母 S. cerevisiae 12geneΔ0HSR-iERG6。通过各种 NMR 研究阐明了 1 的结构。结果表明，1 的所有几何异构体均为 E-形式，并且 1 的两个吡喃糖单元均为葡萄糖和半乳糖类型。化合物 1 对革兰氏阳性和革兰氏阴性细菌、酵母和丝状真菌的生长抑制作用较弱。它对多药敏感酵母的生长抑制作用强于野生型酵母。