CEA-LETI, Minatec Campus, Grenoble, France.
Sorbonne Universités, UPMC University Paris 06, CNRS UMR 8237, Laboratoire Jean Perrin, Paris, France.
J Biomed Opt. 2018 Sep;23(9):1-14. doi: 10.1117/1.JBO.23.9.096002.
To approach wide-field optical properties quantification in real heterogeneous biological tissue, we developed a Dual-Step setup that couples a punctual diffuse reflectance spectroscopy (DRS) technique with multispectral imaging (MSI). The setup achieves wide-field optical properties assessment through an initial estimation of scattering with DRS, which is used to estimate absorption with MSI. The absolute quantification of optical properties is based on the ACA-Pro algorithm that has been adapted both for DRS and for MSI. This paper validates the Dual-Step system not only on homogeneous Intralipid phantoms but also on a heterogeneous gelatine phantom with different scattering and absorbing properties.
为了在真实的异质生物组织中实现宽场光学性质定量分析，我们开发了一种双步设置，将单点漫反射光谱（DRS）技术与多光谱成像（MSI）相结合。该设置通过 DRS 对散射进行初步估计，从而实现宽场光学性质评估，然后用 MSI 来估计吸收。光学性质的绝对定量是基于 ACA-Pro 算法，该算法既适用于 DRS，也适用于 MSI。本文不仅在均匀的 Intralipid 体模上验证了双步系统，还在具有不同散射和吸收特性的异质明胶体模上进行了验证。
