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通过限制性酶切指纹图谱鉴定隐蔽质粒pSLT及其在苏格兰沙门氏菌分离株中的发生率研究。

Recognition of the cryptic plasmid, pSLT, by restriction fingerprinting and a study of its incidence in Scottish Salmonella isolates.

作者信息

Brown D J, Munro D S, Platt D J

出版信息

J Hyg (Lond). 1986 Oct;97(2):193-7. doi: 10.1017/s0022172400065268.

DOI:10.1017/s0022172400065268
PMID:3023478
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2083536/
Abstract

The plasmid pSLT is a cryptic plasmid of 60 megadaltons (Md) present in Salmonella typhimurium LT2. We present evidence that it has a characteristic fingerprint when digested with the restriction enzymes PstI and SmaI. Among a representative collection of S. typhimurium isolates it was present in 67% of strains and was widely distributed amongst different phage types (DT) with the exception of DT10 and U285. Furthermore, its prevalence among veterinary isolates was significantly higher than among human isolates. It was not found among any of the 96 strains representative of other salmonella serotypes currently prevalent and thus appears to be serotype-specific.

摘要

质粒pSLT是存在于鼠伤寒沙门氏菌LT2中的一种60兆道尔顿(Md)的隐蔽质粒。我们提供的证据表明,用限制性内切酶PstI和SmaI消化时,它具有特征性指纹图谱。在一组具有代表性的鼠伤寒沙门氏菌分离株中,67%的菌株含有该质粒,并且除了DT10和U285外,它广泛分布于不同的噬菌体类型(DT)中。此外,它在兽医分离株中的流行率显著高于人类分离株。在目前流行的其他沙门氏菌血清型的96株代表性菌株中均未发现该质粒,因此它似乎具有血清型特异性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a344/2083536/ef878da0f206/jhyg00002-0005-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a344/2083536/ef878da0f206/jhyg00002-0005-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a344/2083536/ef878da0f206/jhyg00002-0005-a.jpg

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本文引用的文献

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A new phage-typing scheme for Salmonella typhi-murium.一种用于鼠伤寒沙门氏菌的新噬菌体分型方案。
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Correlation between the presence of a fifty-megadalton plasmid in Salmonella dublin and virulence for mice.都柏林沙门氏菌中五十兆道尔顿质粒的存在与对小鼠的毒力之间的相关性。
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Association of adhesive, invasive, and virulent phenotypes of Salmonella typhimurium with autonomous 60-megadalton plasmids.
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Identification and sequence analysis of lpfABCDE, a putative fimbrial operon of Salmonella typhimurium.鼠伤寒沙门氏菌假定菌毛操纵子lpfABCDE的鉴定与序列分析
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The distribution of plasmids among a representative collection of Scottish strains of Salmonellae.苏格兰沙门氏菌代表性菌株集合中质粒的分布情况。
J Hyg (Lond). 1986 Oct;97(2):199-204. doi: 10.1017/s002217240006527x.
6
Old and new techniques together resolve a problem of infection by Salmonella typhimurium.新旧技术共同解决了鼠伤寒沙门氏菌感染的问题。
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Linkage map of Salmonella typhimurium, edition VII.鼠伤寒沙门氏菌连锁图谱,第七版。
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8
Correlation between the presence of sequences homologous to the vir region of Salmonella dublin plasmid pSDL2 and the virulence of twenty-two Salmonella serotypes in mice.都柏林沙门氏菌质粒pSDL2的vir区域同源序列的存在与二十二种沙门氏菌血清型在小鼠体内的毒力之间的相关性。
Infect Immun. 1990 May;58(5):1180-5. doi: 10.1128/iai.58.5.1180-1185.1990.
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Isolation of the replication and partitioning regions of the Salmonella typhimurium virulence plasmid and stabilization of heterologous replicons.鼠伤寒沙门氏菌毒力质粒复制与分配区域的分离及异源复制子的稳定化
J Bacteriol. 1990 Sep;172(9):5266-77. doi: 10.1128/jb.172.9.5266-5277.1990.
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Conservation of Salmonella typhimurium virulence plasmid maintenance regions among Salmonella serovars as a basis for plasmid curing.鼠伤寒沙门氏菌毒力质粒维持区域在沙门氏菌血清型间的保守性作为质粒消除的基础。
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Infect Immun. 1982 Nov;38(2):476-86. doi: 10.1128/iai.38.2.476-486.1982.
4
Molecular relationships between virulence plasmids of Salmonella serotypes typhimurium and dublin and large plasmids of other Salmonella serotypes.鼠伤寒沙门氏菌和都柏林沙门氏菌毒力质粒与其他沙门氏菌血清型大质粒之间的分子关系。
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Pleiotropic effects of de-repressed bacterial sex factors on colicinogeny and cell wall structure.去阻遏细菌性因子对产大肠杆菌素及细胞壁结构的多效性作用
Mol Gen Genet. 1970;109(1):57-68. doi: 10.1007/BF00334046.
6
Molecular studies of an fi+ plasmid from strains of Salmonella typhimurium.鼠伤寒沙门氏菌菌株中一种Fi+质粒的分子研究。
Mol Gen Genet. 1973 Nov 2;126(2):143-51. doi: 10.1007/BF00330989.
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Fertility inhibition in strains of Salmonella typhimurium.
Mol Gen Genet. 1972;118(1):79-84.
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The plasmid of Salmonella typhimurium LT2.
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Epidemiology of virulence-associated plasmids and outer membrane protein patterns within seven common Salmonella serotypes.七种常见沙门氏菌血清型中毒力相关质粒及外膜蛋白模式的流行病学研究
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Restriction enzyme fingerprinting of enterobacterial plasmids: a simple strategy with wide application.肠道细菌质粒的限制性酶切指纹图谱:一种应用广泛的简单策略。
J Hyg (Lond). 1986 Oct;97(2):205-10. doi: 10.1017/s0022172400065281.