Popoff M Y, Miras I, Coynault C, Lasselin C, Pardon P
Ann Microbiol (Paris). 1984 May-Jun;135A(3):389-98. doi: 10.1016/s0769-2609(84)80080-0.
All studied isolates of Salmonella serotypes abortusovis (16 strains), enteritidis (30 strains), paratyphi C (29 strains), and 2 out of 10 isolates of serotype newport harboured large 54-76-Kb plasmids. No such plasmids were found in the following serotypes: agona, bovismorbificans, heidelberg, infantis, panama, paratyphi A, paratyphi B, saintpaul, senftenberg and typhi. These plasmids and the virulence-associated plasmids of Salmonella serotypes typhimurium and dublin were compared at the molecular level. Plasmids from the same serotype usually showed similar HindIII endonuclease patterns. Plasmids from different serotypes displayed markedly different cleavage patterns. Using the 3H-labelled plasmid from serotype typhimurium strain C5 as a probe, nitrocellulose filter hybridization showed that all these plasmids shared homologous sequences distributed throughout the plasmid molecule. With the S1-nuclease method, all plasmids were 61 to 88% related to the virulence plasmid of serotype typhimurium strain C5. The large plasmids in Salmonella serotypes abortusovis, enteritidis, paratyphi C, newport and the virulence-associated plasmids in serotypes typhimurium and dublin thus constitute a single group of homology and represent a family of related plasmids. We suggest that this plasmid group may contribute to the pathogenic potential of host serotypes.
所有研究的流产型沙门氏菌血清型（16株）、肠炎型沙门氏菌血清型（30株）、丙型副伤寒沙门氏菌血清型（29株）的分离株，以及10株纽波特血清型分离株中的2株，都含有54 - 76kb的大质粒。在以下血清型中未发现此类质粒：阿哥纳血清型、牛病沙门氏菌血清型、海德堡血清型、婴儿沙门氏菌血清型、巴拿马血清型、甲型副伤寒沙门氏菌血清型、乙型副伤寒沙门氏菌血清型、圣保罗血清型、森夫滕贝格血清型和伤寒沙门氏菌血清型。对这些质粒与鼠伤寒沙门氏菌血清型和都柏林沙门氏菌血清型的毒力相关质粒进行了分子水平比较。来自同一血清型的质粒通常显示出相似的HindIII内切酶图谱。来自不同血清型的质粒显示出明显不同的切割图谱。以鼠伤寒沙门氏菌C5株的3H标记质粒为探针，硝酸纤维素滤膜杂交显示所有这些质粒都具有分布在整个质粒分子中的同源序列。用S1核酸酶法测定，所有质粒与鼠伤寒沙门氏菌C5株的毒力质粒的相关性为61%至88%。因此，流产型沙门氏菌血清型、肠炎型沙门氏菌血清型、丙型副伤寒沙门氏菌血清型、纽波特血清型中的大质粒以及鼠伤寒沙门氏菌血清型和都柏林沙门氏菌血清型中的毒力相关质粒构成了一个单一的同源组，代表了一组相关质粒家族。我们认为，这个质粒组可能对宿主血清型的致病潜力有贡献。
