División de Ciencias Naturales y Exactas Campus Guanajuato, Departamento de Biología, Universidad de Guanajuato, Noria Alta S/N, Col. Noria Alta, 36000, Guanajuato, Guanajuato, México.
Mycopathologia. 2011 Apr;171(4):251-9. doi: 10.1007/s11046-010-9372-8. Epub 2010 Nov 17.
Sporothrix schenckii is the etiologic agent of sporotrichosis. This fungal infection is an emerging disease potentially fatal in immunocompromised patients. The adhesion to host cells is a crucial early event related with the dissemination of pathogens. In order to clarify the mechanisms of adhesion of S. schenckii yeast cell to epithelial cells, we studied the biochemical basis of this process. The electrophoretic analysis of cell wall protein from S. schenckii coupled at ConA and stained with HRP, revealed nine different proteins with MW ≥ 180, 115, 90, 80, 58, 40, 36, 22 and 18 kDa. Using ligand-like assay with biotinylated S. schenckii surface proteins, five proteins with MW ≥ 190, 180, 115, 90 and 80 kDa which have affinity to epithelial cells were identified. The adhesion of yeast to epithelial monolayer was significantly inhibited when S. schenckii was pretreated with concanavalinA (ConA) and wheat germ agglutinin (WGA) lectins, alkali, periodate, trypsin, endoglycosidase H (EndoH), salt solutions and detergents. The ability of adhesion of S. schenckii yeast was recovered by blocking the lectin with sugar complementary. These data suggest that surface glycoprotein with mannose and glucose residue could be participate in the process of fungal adhesion to epithelial cells.
申克孢子丝菌是孢子丝菌病的病原体。这种真菌感染是一种潜在致命的免疫功能低下患者的新兴疾病。与宿主细胞的黏附是与病原体传播相关的早期关键事件。为了阐明 S. schenckii 酵母细胞与上皮细胞黏附的机制，我们研究了这个过程的生化基础。与 ConA 偶联并被 HRP 染色的 S. schenckii 细胞壁蛋白的电泳分析显示，有 9 种不同的蛋白，MW≥180、115、90、80、58、40、36、22 和 18 kDa。使用生物素化 S. schenckii 表面蛋白的配体样测定法，鉴定出 5 种 MW≥190、180、115、90 和 80 kDa 的蛋白，它们与上皮细胞具有亲和力。当 S. schenckii 用伴刀豆球蛋白 A（ConA）和麦胚凝集素（WGA）凝集素、碱、高碘酸盐、胰蛋白酶、内切糖苷酶 H（EndoH）、盐溶液和去污剂预处理时，酵母与上皮单层的黏附显著受到抑制。S. schenckii 酵母黏附能力可通过糖互补阻断凝集素来恢复。这些数据表明，具有甘露糖和葡萄糖残基的表面糖蛋白可能参与真菌与上皮细胞黏附的过程。
