Wang Tong, Dai Yuzhu, Lu Wei, Zhou Huajun, Chen Yu, Xu Xujian, Sun Changgui, Cheng Jun
Faculty of Graduate Studies, Bengbu Medical College, Bengbu Department of Clinical Laboratory, The 117th Hospital of PLA State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China Department of Biotechnology, The University of Tokyo, Tokyo, Japan.
Medicine (Baltimore). 2018 Sep;97(38):e12201. doi: 10.1097/MD.0000000000012201.
This study aimed to investigate hepatitis B virus (HBV) infection in military personnel in eastern China, which will provide a basis for the prevention of HBV infection.A total of 15,508 soldiers and 2386 officers were recruited from military camps in eastern China. The markers, deoxyribonucleic acid, serotypes, and genotypes of HBV in serum were detected and analyzed.Hepatitis B surface antigen (HBsAg) positive rate was 0.44% in soldiers, in whom the low-level HBsAg accounted for 88.24%. The HBsAg positive rate was 1.72% in officers in whom the low-level HBsAg accounted for 12.20%. There were significant differences in the prevalence of high-level and low-level HBsAg, HBV serotypes, HBV DNA positive rate, and mean log HBV DNA between officers and soldiers (P < .05). Compared with the conventional method for HBV DNA extraction, the enrichment method for HBV DNA extraction could significantly improve the positive rate and quantification of HBV DNA by real-time fluorescence quantitative polymerase chain reaction (P < .05). Sequencing of S gene in HBV was used for the determination of serotype and genotype of HBV. The sequencing success rate was significantly different between soldiers and officers (P < .05) as well as between high-level HBsAg group and low-level HBsAg group (P < .05). Significant difference was also observed in the genotype distribution between soldiers and officers (P < .05).HBV infection displays a low prevalence and a low epidemic state, and the prevalence of low-level HBsAg is higher in soldiers. We should pay attention to improve the quality of conscription examination as well as emphasize the surveillance, prevention, and protection of HBV infection in military officers.
本研究旨在调查中国东部地区军事人员的乙型肝炎病毒（HBV）感染情况，为预防HBV感染提供依据。共从中国东部军营招募了15508名士兵和2386名军官。对血清中HBV的标志物、脱氧核糖核酸、血清型和基因型进行了检测和分析。士兵中乙型肝炎表面抗原（HBsAg）阳性率为0.44%，其中低水平HBsAg占88.24%。军官中HBsAg阳性率为1.72%，其中低水平HBsAg占12.20%。军官和士兵在高水平和低水平HBsAg的患病率、HBV血清型、HBV DNA阳性率以及平均log HBV DNA方面存在显著差异（P<0.05）。与传统的HBV DNA提取方法相比，HBV DNA提取的富集方法可显著提高实时荧光定量聚合酶链反应检测HBV DNA的阳性率和定量（P<0.05）。采用HBV中S基因测序来确定HBV的血清型和基因型。士兵和军官之间以及高水平HBsAg组和低水平HBsAg组之间的测序成功率存在显著差异（P<0.05）。士兵和军官之间的基因型分布也存在显著差异（P<0.05）。HBV感染呈现低患病率和低流行状态，士兵中低水平HBsAg的患病率较高。我们应注意提高征兵检查质量，并加强对军官HBV感染的监测、预防和保护。
