Tani-Matsuhana Saori, Vieceli Felipe Monteleone, Gandhi Shashank, Inoue Kunio, Bronner Marianne E
Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA; Department of Biology, Graduate School of Science, Kobe University, Kobe 657-8501, Japan.
Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
Dev Biol. 2018 Dec 1;444 Suppl 1(Suppl 1):S209-S218. doi: 10.1016/j.ydbio.2018.09.015. Epub 2018 Sep 17.
The cardiac neural crest originates in the caudal hindbrain, migrates to the heart, and contributes to septation of the cardiac outflow tract and ventricles, an ability unique to this neural crest subpopulation. Here we have used a FoxD3 neural crest enhancer to isolate a pure population of cardiac neural crest cells for transcriptome analysis. This has led to the identification of transcription factors, signaling receptors/ligands, and cell adhesion molecules upregulated in the early migrating cardiac neural crest. We then functionally tested the role of one of the upregulated transcription factors, MafB, and found that it acts as a regulator of Sox10 expression specifically in the cardiac neural crest. Our results not only reveal the genome-wide profile of early migrating cardiac neural crest cells, but also provide molecular insight into what makes the cardiac neural crest unique.
心脏神经嵴起源于后脑尾部，迁移至心脏，并参与心脏流出道和心室的分隔，这是该神经嵴亚群独有的能力。在此，我们利用FoxD3神经嵴增强子分离出纯的心脏神经嵴细胞群体用于转录组分析。这使得我们鉴定出在早期迁移的心脏神经嵴中上调的转录因子、信号受体/配体和细胞黏附分子。然后，我们对上调的转录因子之一MafB的作用进行了功能测试，发现它 specifically在心脏神经嵴中作为Sox10表达的调节因子发挥作用。我们的结果不仅揭示了早期迁移的心脏神经嵴细胞的全基因组概况，还为使心脏神经嵴独特的因素提供了分子层面的见解。
