Kraus A L, Bernstein I A
J Toxicol Environ Health. 1986;19(4):541-54. doi: 10.1080/15287398609530951.
Removal from adipose tissue is an important first step in ultimate removal of many lipophilic xenobiotics from the body. This study concerned the elucidation of mechanisms by which hexabromobiphenyl (HBB) was deposited in and removed from adipocytes. Adipocytes derived from the 3T3L1 cell line of mouse fibroblasts were used to conduct studies in vitro. Results support the idea that HBB enters the 3T3L1 adipocyte via passive diffusion. A plot of the velocity of uptake versus concentration was linear, the uptake of HBB does not appear to be energy dependent, and structurally similar biphenyls did not cause an inhibition of uptake. A linear relationship between the quantities of triglyceride and HBB in the cells was found during both uptake of HBB in lipogenesis and removal of HBB in lipolysis (r greater than 0.98). This supports the contention that the quantity of triglyceride in the cells has a strong influence on the movement of HBB between adipocytes and surrounding pseudoblood. Evidence has been presented that is consistent with the hypothesis that HBB moves freely across the adipocyte membrane and is sequestered in either cells or medium according to its relative solubility in these compartments. Methods to increase the removal of HBB from adipocytes have been proposed.
从脂肪组织中清除是最终从体内清除许多亲脂性外源性物质的重要第一步。本研究关注六溴联苯（HBB）在脂肪细胞中沉积和清除的机制。从小鼠成纤维细胞的3T3L1细胞系衍生而来的脂肪细胞用于体外研究。结果支持HBB通过被动扩散进入3T3L1脂肪细胞的观点。摄取速度与浓度的关系图呈线性，HBB的摄取似乎不依赖能量，且结构相似的联苯不会抑制摄取。在脂肪生成过程中HBB的摄取和脂肪分解过程中HBB的清除过程中，细胞内甘油三酯与HBB的量之间均发现了线性关系（r大于0.98）。这支持了这样的观点，即细胞内甘油三酯的量对HBB在脂肪细胞与周围假血液之间的移动有很大影响。已有证据与以下假设一致，即HBB可自由穿过脂肪细胞膜，并根据其在这些区室中的相对溶解度而被隔离在细胞或培养基中。已提出增加从脂肪细胞中清除HBB的方法。
