Alexandersen S, Bloom M E
J Virol. 1987 Jan;61(1):81-6. doi: 10.1128/JVI.61.1.81-86.1987.
We studied different parameters during the development of acute interstitial pneumonia in mink kits caused by neonatal infection with Aleutian disease virus (ADV). When histological lesions, presence of intranuclear inclusion bodies, and intranuclearly localized ADV antigen were correlated with levels of single-stranded virion and duplex replicative forms of ADV DNA in the different tissues, it was concluded that the lung, probably alveolar type II cells, is the major primary target for viral replication and cytopathology. The presence of the duplex dimeric replicative-form DNA, a strong marker of parvovirus replication, was also observed in low amount in the mesenteric lymph node, suggesting replication of ADV in this organ, although no viral cytopathology could be demonstrated. Moreover, a few intranuclear inclusion bodies were demonstrated in kidney and liver from affected kits, but intranuclearly localized ADV antigen could not be demonstrated in liver sections, and neither could duplex dimer replicative-form DNA, suggesting that these organs are nevertheless not a major site of ADV replication. When the data were compared with results previously reported for ADV-infected adult mink and ADV-infected permissive cell cultures, the data suggested that the pattern of ADV replication in alveolar type II cells is similar to that seen in infected cell cultures but that the replication in the other kit organs resembles the restricted pattern seen in adult mink.
我们研究了水貂幼崽因感染阿留申病病毒（ADV）而引发急性间质性肺炎过程中的不同参数。当将组织学病变、核内包涵体的存在以及核内定位的ADV抗原与不同组织中ADV DNA的单链病毒粒子和双链复制形式的水平相关联时，得出的结论是，肺，可能是II型肺泡细胞，是病毒复制和细胞病理学的主要初始靶标。在肠系膜淋巴结中也少量观察到双链二聚体复制形式DNA的存在，这是细小病毒复制的一个强标志物，提示ADV在该器官中复制，尽管未发现病毒细胞病理学变化。此外，在患病幼崽的肾脏和肝脏中发现了一些核内包涵体，但在肝脏切片中未检测到核内定位的ADV抗原，也未检测到双链二聚体复制形式DNA，这表明这些器官并非ADV复制的主要部位。当将这些数据与先前报道的ADV感染成年水貂和ADV感染的允许性细胞培养物的结果进行比较时，数据表明II型肺泡细胞中ADV的复制模式与感染细胞培养物中的相似，但幼崽其他器官中的复制类似于成年水貂中所见的受限模式。
