Alexandersen S
Vet Pathol. 1986 Sep;23(5):579-88. doi: 10.1177/030098588602300506.
Organ homogenates from kits that died of interstitial pneumonia were inoculated into adult Aleutian disease virus (ADV)-negative mink and shown to contain infectious ADV. Acute interstitial pneumonia was experimentally reproduced with the organ homogenate but only by inoculation of newborn kits born from ADV-negative dams. Older kits and kits from ADV-positive dams did not develop interstitial pneumonia, but later developed the classic form of Aleutian disease. Electron microscopic examination was done on purified suspensions of defined ADV isolates and on purified organ homogenates from kits with spontaneous or experimental interstitial pneumonia. In kits from both groups a virus, morphologically resembling the defined ADV isolates, was demonstrated. Findings of intranuclear inclusion bodies and intranuclear ADV antigen in alveolar type-II cells in affected lungs and the lack of immunologically mediated lesions suggest that lung lesions result from primary viral injury to alveolar type-II cells. Experiments also showed that infection of dams with ADV before pregnancy decreased the number of kits per mated dam and infection with ADV in mid-pregnancy caused fetal death, fetal resorption, or abortion.
将死于间质性肺炎的水貂的器官匀浆接种到成年的阿留申病病毒（ADV）阴性水貂体内，结果显示其中含有传染性ADV。用该器官匀浆可通过实验复制出急性间质性肺炎，但仅能通过接种来自ADV阴性母貂的新生水貂实现。年龄较大的水貂以及来自ADV阳性母貂的水貂不会患上间质性肺炎，但随后会发展为典型的阿留申病。对明确的ADV分离株的纯化悬液以及来自患有自发性或实验性间质性肺炎的水貂的纯化器官匀浆进行了电子显微镜检查。在两组水貂中均证实存在一种形态上与明确的ADV分离株相似的病毒。在受影响肺部的II型肺泡细胞中发现核内包涵体和核内ADV抗原，且缺乏免疫介导的病变，这表明肺部病变是由病毒对II型肺泡细胞的原发性损伤所致。实验还表明，怀孕前母貂感染ADV会减少每只受孕母貂所产仔貂的数量，而怀孕中期感染ADV会导致胎儿死亡、胎儿吸收或流产。
