Thader Biotechnology SL, Campus de Espinardo, Murcia, Spain.
Departamento de Biología Vegetal (Botánica), Facultad de Biología, Universidad de Murcia, Campus de Espinardo, Murcia, Spain.
PLoS One. 2019 Jul 10;14(7):e0219300. doi: 10.1371/journal.pone.0219300. eCollection 2019.
Terfezia claveryi Chatin is a mycorrhizal fungus that forms ectendomycorrhizal associations with plants of Helianthemum genus. Its appreciated edibility and drought resistance make this fungus a potential alternative crop in arid and semiarid areas of the Mediterranean region. In order to increase the knowledge about the biology of this fungus in terms of mycorrhiza formation and response to drought stress, a catalase from T. claveryi (TcCAT-1) has been purified to apparent homogeneity and biochemically characterized; in addition, the expression pattern of this enzyme during different stages of T. claveryi biological cycle and under drought stress conditions are reported. The results obtained, together with the phylogenetic analysis and homology modeling, indicate that TcCAT-1 is a homotetramer large subunit size monofunctional-heme catalase belonging to Clade 2. The highest expression of this enzyme occurs in mature mycorrhiza, revealing a possible role in mycorrhiza colonization, but it is not upregulated under drought stress. However, the H2O2 content of mycorrhizal plants submitted to drought stress is lower than in well watered treatments, suggesting that mycorrhization improves the plant's oxidative stress response, although not via TcCAT-1 upregulation.
克莱维酵母（Terfezia claveryi Chatin）是一种与半日花属（Helianthemum）植物形成外生菌根共生关系的共生真菌。其可食用性和耐旱性使其成为地中海干旱和半干旱地区潜在的替代作物。为了增加对该真菌在菌根形成和对干旱胁迫响应方面的生物学知识，本研究从克莱维酵母（T. claveryi）中纯化出一种过氧化氢酶（TcCAT-1），并对其进行了生化特性分析；此外，还报告了该酶在不同发育阶段和干旱胁迫条件下的表达模式。获得的结果，结合系统发育分析和同源建模，表明 TcCAT-1 是一种同四聚体的大亚基大小的单功能血红素过氧化氢酶，属于 Clade 2。该酶在成熟菌根中的表达最高，表明其可能在菌根定殖中发挥作用，但在干旱胁迫下并未上调。然而，与未受干旱胁迫处理的植株相比，干旱胁迫下菌根植株的 H2O2 含量较低，这表明菌根化提高了植物的氧化应激响应，尽管这不是通过 TcCAT-1 的上调实现的。
