Fichou Dimitri, Morlock Gertrud E
Chair of Food Science, Institute of Nutritional Science, and Interdisciplinary Research Center (IFZ) , Justus Liebig University Giessen , Heinrich-Buff-Ring 26-32 , 35392 Giessen , Germany.
Anal Chem. 2018 Nov 6;90(21):12647-12654. doi: 10.1021/acs.analchem.8b02866. Epub 2018 Oct 8.
Current high-performance-thin-layer-chromatography instrumentation is offline and stepwise automated. However, moderate miniaturization offers many advantages and together with the transfer of modern print and media technologies to the field of chromatography (office chromatography) it opens up new avenues. This is demonstrated in an all-in-one open-source system developed for planar chromatography and especially for ultrathin-layer chromatography. Using an InkShield board to control a thermal inkjet cartridge, picoliter drops were printed at a resolution of 96 dpi on the adsorbent layer. Using Marlin, a popular firmware in 3D printing, Cartesian movement of the print head was made possible for full control of the printing process. Open-source software was developed to control the device in each operation step. Sample solutions and mobile phase were inkjet-printed, exemplarily shown for the analysis of dye- or paraben-mixture solutions. Light-emitting diodes (LEDs) were investigated for documentation. For example, deep UV LEDs gave access to 254 nm light, and RGB LEDs gave access to the visible-light range. Calibration functions with correlation coefficients superior to 0.999 were obtained by videodensitometry. The developed modular open-source hardware was compact (26 × 31 × 26 cm), light (<3 kg), and affordable (€810). For the given analyses, the footprint of current instrumentation needed was miniaturized by a factor of 9. The highly reduced material design complies with green chemistry and lean laboratory. The design and instruction to reproduce the all-in-one open-source system were made freely available at https://github.com/OfficeChromatography . It is intended to boost progress and understanding by the nature of do it yourself.
当前的高性能薄层色谱仪器是离线且逐步自动化的。然而，适度的小型化具有诸多优势，并且随着现代印刷和媒体技术向色谱领域的转移（办公色谱），它开辟了新的途径。这在一个为平面色谱，特别是超薄层色谱开发的一体化开源系统中得到了证明。使用一个InkShield板来控制一个热喷墨墨盒，以96 dpi的分辨率在吸附剂层上打印皮升液滴。利用3D打印中一种流行的固件Marlin，实现了打印头的笛卡尔运动，从而能够完全控制打印过程。开发了开源软件来控制设备的每个操作步骤。对样品溶液和流动相进行了喷墨打印，以染料或对羟基苯甲酸酯混合溶液的分析为例进行了展示。研究了发光二极管（LED）用于记录。例如，深紫外LED可提供254 nm的光，而RGB LED可提供可见光范围的光。通过视频密度测定法获得了相关系数优于0.999的校准函数。所开发的模块化开源硬件紧凑（26×31×26 cm）、轻便（<3 kg）且价格实惠（810欧元）。对于给定的分析，所需的当前仪器占地面积缩小了9倍。高度简化的材料设计符合绿色化学和精益实验室的要求。该一体化开源系统的设计和复制说明可在https://github.com/OfficeChromatography上免费获取。其目的是通过自己动手的性质来推动进步和理解。
