Woortman Dirk Volker, Haack Martina, Mehlmer Norbert, Brück Thomas B
Technical University of Munich, Werner Siemens-Chair of Synthetic Biotechnology (WSSB), Lichtenbergstraße 4, 85748 Garching, Germany.
ACS Omega. 2020 May 6;5(19):11147-11150. doi: 10.1021/acsomega.0c01096. eCollection 2020 May 19.
Additive manufacturing, known as three-dimensional (3D) printing technologies, has revolutionized production in all domains of science and technology. Although 3D printing has a high impact on research and development, its capacity to implement low-cost, flexible, and robust sample handling automation has not been exploited in full. To this end, we have created a low-cost, robust, and easy-to-utilize kit to transform an off-the-shelf fused deposition modeling 3D printer to a thin layer chromatography (TLC) sample application device. Our technology solution improves TLC convenience when higher throughput of the established method is required. The developed dual-needle sprayer allows simple and exceptionally robust automatic sample application. The device is especially well-suited for high-performance TLC-assisted method selection in counter-current chromatography. A step-by-step guide and list of required parts, including 3D printable files with instruction, can be obtained from the Supporting Information for research usage and open development.
增材制造，即三维（3D）打印技术，已经彻底改变了所有科技领域的生产方式。尽管3D打印对研发有很大影响，但其实现低成本、灵活且强大的样品处理自动化的能力尚未得到充分利用。为此，我们创建了一个低成本、坚固且易于使用的套件，用于将现成的熔融沉积建模3D打印机转变为薄层色谱（TLC）样品施加装置。当需要提高既定方法的通量时，我们的技术解决方案提高了TLC的便利性。所开发的双针喷雾器允许进行简单且极其坚固的自动样品施加。该装置特别适用于逆流色谱中高性能TLC辅助的方法选择。可以从支持信息中获取详细的操作指南和所需部件列表，包括带有说明的3D可打印文件，以供研究使用和开放开发。
