Duman Muhammed, Saticioglu Izzet Burcin, Janda J Michael, Altun Soner
Faculty of Veterinary Medicine, Aquatic Animal Disease Department, Uludag University, Bursa, Turkey.
Faculty of Veterinary Medicine, Aquatic Animal Disease Department, Erciyes University, Kayseri, Turkey.
J Fish Dis. 2018 Dec;41(12):1843-1857. doi: 10.1111/jfd.12896. Epub 2018 Sep 21.
The aims of this study were to determine the prevalence and phylogenetic relationship of motile Aeromonas spp. that might be pathogenic species for rainbow trout in infected/mix infection cases (based upon different outbreaks on fish farms). A total of 99 motile Aeromonas isolates (and three reference strains) were analysed that were isolated from four different fish species in different sizes of fish (0.1-3,000 g), different months and water temperatures (6.1-21.2°C). The biochemical characteristics of the isolates were determined using conventional tests and a rapid test kit. Additionally, molecular identification was performed using the gyrB housekeeping gene region and with glycerophospholipid-cholesterol acyltransferase polymerase chain reaction (GCAT-PCR). The sequencing results obtained from the gyrB gene region were deposited in the GenBank database, and phylogenetic relationships were determined with the BioNumerics 7.6 database. Nearly half of the Aeromonas isolates that were isolated from rainbow trout showing signs of disease were determined to be possible infectious agents. Aeromonas species exhibit biochemical variability for many characters, so some Aeromonas species tested negative for GCAT-PCR despite that this test was created especially for Aeromonas identification. The phylogenetic tree based upon gyrB contained 10 different phylogroups that were based on 96% cut-off value in gyrB gene region.
本研究的目的是确定运动性气单胞菌属的流行情况及其系统发育关系，这些菌在感染/混合感染病例中可能是虹鳟鱼的致病物种（基于养鱼场的不同疫情）。共分析了99株运动性气单胞菌分离株（以及三株参考菌株），它们是从不同大小（0.1 - 3000克）、不同月份和水温（6.1 - 21.2°C）的四种不同鱼类中分离得到的。使用传统试验和快速检测试剂盒确定分离株的生化特性。此外，使用gyrB管家基因区域和甘油磷脂 - 胆固醇酰基转移酶聚合酶链反应（GCAT - PCR）进行分子鉴定。从gyrB基因区域获得的测序结果存入GenBank数据库，并使用BioNumerics 7.6数据库确定系统发育关系。从出现疾病症状的虹鳟鱼中分离出的气单胞菌分离株中，近一半被确定为可能的感染源。气单胞菌属在许多特征上表现出生化变异性，因此尽管GCAT - PCR是专门为气单胞菌鉴定而创建的，但一些气单胞菌物种在该检测中呈阴性。基于gyrB构建 phylogenetic tree包含10个不同的系统发育组，这些组基于gyrB基因区域96%的截止值。 （注：phylogenetic tree一般译为“系统发育树”，此处保留英文未翻译，可能是原文存在错误表述，若要准确翻译需结合专业知识进一步确认其确切所指。）
