Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, China.
Shenzhen Key Laboratory of Marine Bioresource and Eco-Environmental Science, Guangdong Engineering Research Center for Marine Algal Biotechnology, College of Life Science and Oceanography, Shenzhen University, Shenzhen, China.
J Biophotonics. 2019 Feb;12(2):e201800263. doi: 10.1002/jbio.201800263. Epub 2018 Oct 5.
Three-photon microscopy excited at the 1700-nm window enables deep-tissue penetration. However, the refractive indices of commonly used immersion oils, and the resultant pulse broadening are not known, preventing imaging optimization. Here, we demonstrate detailed characterization of the refractive index, pulse broadening and distortion for excitation pulses at this window for commonly used immersion oils. On the physical side, we uncover that absorption, rather than material dispersion, is the main cause of pulse broadening and distortion. On the application side, comparative three-photon imaging results indicate that 1600-nm excitation yields 5 times higher three-photon signal than 1690-nm excitation.
三光子显微镜在 1700nm 窗口激发，实现了深层组织穿透。然而，常用浸没油的折射率以及由此产生的脉冲展宽尚不清楚，这阻碍了成像的优化。在这里，我们展示了对常用浸没油在该窗口的激发脉冲的折射率、脉冲展宽和失真的详细特性。从物理方面来看，我们揭示了脉冲展宽和失真的主要原因是吸收，而不是材料色散。在应用方面，比较三光子成像结果表明，1600nm 激发产生的三光子信号比 1690nm 激发高 5 倍。
