Bogenhagen D F, Yoza B K
Mol Cell Biol. 1986 Jul;6(7):2543-50. doi: 10.1128/mcb.6.7.2543-2550.1986.
The mitochondrial RNA polymerase from Xenopus laevis oocytes was partially purified by heparin-Sepharose chromatography and phosphocellulose chromatography. This RNA polymerase preparation specifically initiated the transcription of X. laevis mitochondrial DNA (mtDNA) from two bidirectional promoters contained within a 123-base-pair segment of the mtDNA between the heavy-strand replication origin and the rRNA cistrons. Transcription in vitro initiated from precisely the same start sites previously mapped as initiation sites for transcription in vivo. At each of the four sites, initiation occurred within a conserved nucleotide sequence, ACPuTTATA. This consensus sequence is not related to promoters for transcription of human mtDNA.
非洲爪蟾卵母细胞的线粒体RNA聚合酶通过肝素-琼脂糖层析和磷酸纤维素层析进行了部分纯化。这种RNA聚合酶制剂能特异性地从线粒体DNA（mtDNA）重链复制起点和rRNA顺反子之间123个碱基对片段中包含的两个双向启动子起始非洲爪蟾mtDNA的转录。体外转录精确地从先前确定为体内转录起始位点的相同起始位点开始。在这四个位点中的每一个位点，起始都发生在一个保守的核苷酸序列ACPuTTATA内。这个共有序列与人类mtDNA转录的启动子无关。
