College of Animal Science and Technology, Nanjing Agricultural University, Jiangsu 210095, Nanjing, P. R. China.
Poult Sci. 2019 Feb 1;98(2):887-895. doi: 10.3382/ps/pey434.
This experiment was conducted to evaluate the effects of bile acids (BAs) on the growth performance and lipid metabolism of broilers fed with different energy level diets. 480 one-day-old Arbor Acres broilers (45.01 ± 0.26 g) were allotted to a 2 × 2 factorial design with 2 levels of energy (basal or high-energy level) and 2 levels of BAs (with or without BAs supplementation), resulting in 4 groups of 8 replicates; the experiment lasted 42 d. High-energy diets decreased the feed/gain ratio (F/G) from 1 to 21 d (P < 0.05), and increased the liver index and abdominal fat percentage at 42 d (P < 0.05). The serum total triglyceride (TG) and high-density lipoprotein cholesterol at 42 d were increased by high-energy diets (P < 0.05), while the hepatic lipoprotein lipase (LPL) activity at 21 and 42 d was decreased (P < 0.05). BAs supplementation increased the body weight at 21 d and decreased the F/G during entire period (P < 0.05), as well as improved the carcass quality reflected by decreased abdominal fat percentage at 42 d and increased breast muscle percentage at 21 and 42 d (P < 0.05). The serum TG at 21 and 42 d were decreased by BAs (P < 0.05), and the hepatic LPL activity at 42 d was increased (P < 0.05). In addition, high-energy diets increased the expression of sterol regulatory element binding transcription factor 1, acetyl-CoA carboxylase, and fatty acid synthase (P < 0.05), while BAs diets decreased these genes expression (P < 0.05). Moreover, BAs supplementation also increased the expression of carnitine palmitoyltransferase 1 (P < 0.05), which was increased in high-energy groups (P < 0.05). In conclusion, BAs supplementation could increase growth performance, elevate carcass quality, and improve lipid metabolism in broilers.
本实验旨在评估胆汁酸（BAs）对不同能量水平日粮喂养肉鸡生长性能和脂质代谢的影响。选用 480 只 1 日龄爱拔益加肉鸡（45.01±0.26 g），采用 2×2 完全析因设计，设置 2 个能量水平（基础或高能水平）和 2 个 BAs 水平（添加或不添加 BAs），每个处理 8 个重复，试验持续 42 d。高能日粮降低了 1~21 d 的料重比（F/G）（P<0.05），增加了 42 d 的肝脏指数和腹脂率（P<0.05）。高能日粮增加了 42 d 的血清总甘油三酯（TG）和高密度脂蛋白胆固醇（P<0.05），但降低了 21 和 42 d 的肝脂蛋白脂肪酶（LPL）活性（P<0.05）。BAs 添加增加了 21 d 的体重，并降低了整个试验期的 F/G（P<0.05），还改善了 42 d 的腹脂率和 21、42 d 的胸肌率等胴体质量（P<0.05）。BAs 添加降低了 21 和 42 d 的血清 TG（P<0.05），增加了 42 d 的肝 LPL 活性（P<0.05）。此外，高能日粮增加了固醇调节元件结合转录因子 1、乙酰辅酶 A 羧化酶和脂肪酸合酶的表达（P<0.05），而 BAs 日粮降低了这些基因的表达（P<0.05）。此外，BAs 添加还增加了肉碱棕榈酰转移酶 1 的表达（P<0.05），在高能组中也增加了（P<0.05）。综上所述，BAs 添加可提高肉鸡的生长性能，改善胴体质量，改善脂质代谢。
