用离子液体双水相体系从螺旋藻中分离 C-藻蓝蛋白。
Isolation of C-phycocyanin from Spirulina platensis microalga using Ionic liquid based aqueous two-phase system.
机构信息
Department of Chemical Engineering, Graduate School of Biochemical Engineering, Ming Chi University of Technology, New Taipei City, Taiwan.
Bioseparation Research Group, Department of Chemical Engineering and Environmental Engineering, University of Nottingham Malaysia Campus, Selangor Darul Ehsan, Malaysia.
出版信息
Bioresour Technol. 2018 Dec;270:320-327. doi: 10.1016/j.biortech.2018.07.138. Epub 2018 Sep 12.
An aqueous two-phase system (ATPS) with ionic liquids (ILs) was used for the isolate of C-phycocyanin (CPC) from Spirulina platensis microalga. Various imidazolium ILs and potassium salts were studied. The effect of ILs-ATPS on the extraction efficiency of CPC was also studied. The experimental parameters like pH, loading volume, algae concentration, temperature, and alkyl chain length of IL were well-covered in this report. The experimental results showed that the extraction efficiency, the partition coefficient, and the separation factor for CPC were 99%, 36.6, and 5.8, respectively, for an optimal pH value of 7 and a temperature of 308 K. The order of extraction efficiency for CPC using IL-ATPS was: 1-octyl-3-methylimidazolium bromide (C8MIM-Br) > 1-hexyl-3-methylimidazolium bromide (C6MIM-Br) > 1-butyl-3-methylimidazolium bromide (C4MIM-Br). The isolation process followed the pseudo second-order kinetic model and the thermodynamic results were obviously spontaneous.
双水相体系（ATPS）与离子液体（ILs）被用于从螺旋藻微藻中分离藻蓝蛋白（CPC）。本研究考察了不同的咪唑类离子液体和钾盐。还研究了 IL-ATPS 对 CPC 萃取效率的影响。本报告充分考察了实验参数，如 pH 值、加样量、藻浓度、温度和 IL 的烷基链长度。实验结果表明，在最佳 pH 值为 7 和温度为 308 K 时，萃取效率、分配系数和 CPC 的分离因子分别为 99%、36.6 和 5.8。使用 IL-ATPS 萃取 CPC 的效率顺序为：1-辛基-3-甲基咪唑溴（C8MIM-Br）>1-己基-3-甲基咪唑溴（C6MIM-Br）>1-丁基-3-甲基咪唑溴（C4MIM-Br）。分离过程遵循准二级动力学模型，热力学结果明显是自发的。
Zotero 插件