Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, 48175-861, Iran.
Department of Pharmacognosy, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, 48175-861, Iran.
Chin J Integr Med. 2020 Oct;26(10):754-761. doi: 10.1007/s11655-018-2984-4. Epub 2018 Sep 22.
To evaluate the protective effect of Zataria multiflora extract, an antioxidative medicinal plant, against cyclophosphamide (CP)-induced oxidative lung damage in mice.
Mice were intraperitoneally pre-treated with various doses of Zataria multiflora extract (50, 100, 200, and 400 mg/kg) once daily for 7 consecutive days. Animals were then injected with a single 200 mg/kg intraperitoneal dose of CP 1 h after the last administration of O. vulgare. Twenty-four hours later, mice were euthanized, the lungs were immediately removed, and biochemical and histological studies were conducted.
A single dose of CP markedly altered the levels of several biomarkers associated with oxidative stress in lung homogenates. Pretreatment with Zataria multiflora significantly inhibited the elevation of lipid peroxidation level and the depletion in glutathione content, and superoxide dismutase and catalase activities induced by CP in lung. In addition, Zataria multiflora effectively alleviated CP-induced histopathological abnormality and pulmonary damages in mice lung tissues.
The results reveal that Zataria multiflora protects lung tissues from CP-induced toxicity and suggest a role for oxidative stress in the pathogenesis of lung toxicity produced by CP in mice. Because Zataria multiflora has been extensively used as an additive agent and is regarded as safe, it may be used concomitantly as a good supplement for reducing organ toxicity in patients undergoing chemotherapy, besides their consolidated ethnopharmacological uses.
评估唇形科植物香薷（Zataria multiflora）提取物作为一种抗氧化药用植物，对环磷酰胺（CP）诱导的小鼠氧化肺损伤的保护作用。
小鼠每天腹腔内预先用不同剂量的香薷提取物（50、100、200 和 400mg/kg）预处理一次，连续 7 天。最后一次给药 1 小时后，动物腹腔内注射单次 200mg/kg CP。24 小时后，处死小鼠，立即取出肺脏，进行生化和组织学研究。
单次 CP 给药明显改变了肺匀浆中与氧化应激相关的几种生物标志物的水平。香薷提取物预处理显著抑制了 CP 诱导的肺脂质过氧化水平升高、谷胱甘肽含量降低以及超氧化物歧化酶和过氧化氢酶活性的抑制。此外，香薷提取物有效缓解了 CP 诱导的小鼠肺组织的组织病理学异常和肺损伤。
结果表明香薷提取物可保护肺组织免受 CP 诱导的毒性作用，并提示氧化应激在 CP 诱导的小鼠肺毒性发病机制中起作用。由于香薷已广泛用作添加剂，并被认为是安全的，因此除了它们的传统药物用途外，它还可能被用作同时减少化疗患者器官毒性的良好补充剂。
