Key Laboratory for Advanced Technology in Environmental Protection of Jiangsu Province, Yancheng Institute of Technology, Yancheng 224051, China; Department of Industrial Chemistry, College of Applied Sciences, Addis Ababa Science and Technology University, P.O. Box 16417, Addis Ababa, Ethiopia.
Key Laboratory for Advanced Technology in Environmental Protection of Jiangsu Province, Yancheng Institute of Technology, Yancheng 224051, China.
Bioresour Technol. 2018 Dec;270:377-382. doi: 10.1016/j.biortech.2018.09.077. Epub 2018 Sep 15.
Developing a new cellulase-MOF composite system with enhanced stability and reusability for cellulose hydrolysis was aimed. Physical adsorption strategy was employed to fabricate two cellulase composites, and the activity of composite was characterized by hydrolysis of carboxymethyl cellulose. The NH functionalized UiO-66-NH MOF exhibited higher protein loading than the precursor UiO-66, due to the extra anchor sites of NH groups. The immobilized cellulase showed enhanced thermostability, pH tolerance and lifetime. The maximum activity attained at 55 °C could be kept 85% when used at 80 °C, and the residual activities were 72% after ten cycles and 65% after 30 days storage. The abundant NH and COOH groups of MOF adsorb cellulase and enhance its stability, and the resulted heterogeneity offered the opportunity of recovering composite via mild centrifuge. The findings suggest the promising future of developing cellulase-MOF composite with ultrahigh activities and stabilities for practical application.
旨在开发一种新的具有增强的稳定性和可重复使用性的纤维素酶-MOF 复合体系，用于纤维素水解。采用物理吸附策略制备了两种纤维素酶复合材料，并通过羧甲基纤维素的水解来表征复合材料的活性。由于 NH 基团的额外锚固位点，NH 功能化的 UiO-66-NH MOF 比前体 UiO-66 具有更高的蛋白质负载量。固定化纤维素酶表现出增强的热稳定性、pH 耐受性和寿命。在 80°C 下使用时，在 55°C 时达到的最大活性可保持 85%，经过十次循环后残留活性为 72%，经过 30 天储存后残留活性为 65%。MOF 中的丰富的 NH 和 COOH 基团吸附纤维素酶并增强其稳定性，而所得的不均匀性为通过温和离心回收复合材料提供了机会。这些发现表明，开发具有超高活性和稳定性的纤维素酶-MOF 复合材料具有广阔的前景，可用于实际应用。
