Ayee Manuela A A, Levitan Irena
University of Illinois at Chicago, Chicago, IL, United States.
Curr Top Membr. 2018;81:97-123. doi: 10.1016/bs.ctm.2018.07.003. Epub 2018 Aug 17.
The effects of osmotic swelling on key cellular biomechanical properties are explored in this chapter. We present the governing equations and theoretical backgrounds of the models employed to estimate cell membrane tension and elastic moduli from experimental methods, and provide a summary of the prevailing experimental approaches used to obtain these biomechanical parameters. A detailed analysis of the current evidence of the effects of osmotic swelling on membrane tension and elastic moduli is provided. Briefly, due to the buffering effect of unfolding membrane reservoirs, mild hypotonic swelling does not change membrane tension or the adhesion of the membrane to the underlying cytoskeleton. Conversely, osmotic swelling causes the cell membrane envelope to stiffen, measured as an increase in the membrane elastic modulus.
本章探讨了渗透性肿胀对关键细胞生物力学特性的影响。我们介绍了用于从实验方法估算细胞膜张力和弹性模量的模型的控制方程和理论背景，并总结了用于获取这些生物力学参数的主要实验方法。对渗透性肿胀对膜张力和弹性模量影响的现有证据进行了详细分析。简而言之，由于展开的膜储备的缓冲作用，轻度低渗肿胀不会改变膜张力或膜与下层细胞骨架的粘附力。相反，渗透性肿胀会导致细胞膜包膜变硬，表现为膜弹性模量增加。
