Universiti Kuala Lumpur, Malaysian Institute of Chemical and Engineering Technology, Lot 1988 Kawasan Perindustrian Bandar Vendor, Taboh Naning, 78000, Alor Gajah, Melaka, Malaysia.
Universiti Kuala Lumpur, Malaysian Institute of Chemical and Engineering Technology, Lot 1988 Kawasan Perindustrian Bandar Vendor, Taboh Naning, 78000, Alor Gajah, Melaka, Malaysia.
Exp Eye Res. 2019 Jan;178:10-14. doi: 10.1016/j.exer.2018.09.011. Epub 2018 Sep 20.
Microbial keratitis is the infection caused by pathogenic microorganisms that commonly occurs among the contact lens users. Various antimicrobial compounds were coated on contact lenses to kill keratitis causing microorganisms, however these compounds caused several adverse side effects. Hence, the aim of this study is to develop a silicone hydrogel contact lens coated with phomopsidione nanoparticle that inhibit keratitis causing clinical isolates. Phomopsidione nanoparticles were synthesized using polyvinyl alcohol as encapsulant. The nanoparticles showed an average size of 77.45 nm, with neutral surface charge. Two drug release patterns were observed in the drug release profile, which are the initial slow release phase with extended drug release (release rate 46.65 μg/h), and the burst release phase observed on Day 2 (release rate 2224.49 μg/h). This well-regulated drug delivery system enables the control of drug release to meet the therapeutic requirements. On agar diffusion assay, 3 out of 5 test microorganisms were inhibited by phomopsidione nanoparticle coated contact lenses, including two Gram negative bacteria. Besides, all test microorganisms showed at least 99% of growth reduction, with the treatment of the contact lens model. The drug loaded onto the nanoparticles is sufficient to prevent the bacterial growth. In conclusion, this study provides an effective alternative to combat keratitis-causing microorganisms among contact wearers.
微生物角膜炎是由致病微生物引起的感染，常见于隐形眼镜使用者中。为了杀死引起角膜炎的微生物，各种抗菌化合物被涂覆在隐形眼镜上，但这些化合物会引起多种不良反应。因此，本研究旨在开发一种涂有假霜霉素纳米颗粒的硅水凝胶隐形眼镜，以抑制引起角膜炎的临床分离株。假霜霉素纳米颗粒是使用聚乙烯醇作为包封剂合成的。纳米颗粒的平均粒径为 77.45nm，表面带中性电荷。在药物释放曲线中观察到两种药物释放模式，即初始缓慢释放阶段和持续药物释放（释放速率为 46.65μg/h），以及第 2 天观察到的爆发释放阶段（释放速率为 2224.49μg/h）。这种良好调控的药物递送系统能够控制药物释放，以满足治疗需求。在琼脂扩散试验中，涂有假霜霉素纳米颗粒的隐形眼镜抑制了 5 种测试微生物中的 3 种，包括两种革兰氏阴性菌。此外，所有测试微生物的生长减少率均至少为 99%，经过隐形眼镜模型处理后。负载到纳米颗粒上的药物足以防止细菌生长。总之，本研究为隐形眼镜佩戴者提供了一种对抗引起角膜炎的微生物的有效替代方法。
