Siddiqui Ruqaiyyah, Lakhundi Sahreena, Khan Naveed Ahmed
Department of Biological and Biomedical Sciences, Aga Khan University, Karachi, Pakistan.
Department of Biological and Biomedical Sciences, Aga Khan University, Karachi, Pakistan.
Cont Lens Anterior Eye. 2015 Feb;38(1):34-8. doi: 10.1016/j.clae.2014.09.001. Epub 2014 Oct 1.
The aim of this study was to assess the antimicrobial effects of marketed contact lens disinfecting solutions.
Using ISO 14729 Stand-Alone Test for disinfecting solutions, bactericidal, fungicidal and amoebicidal assays of eight different contact lens solutions including: ReNu MultiPlus, DuraPlus, Ultimate Plus, OptiFree Express, Kontex Clean, Kontex Normal, Kontex Multisol extra(+), Kontex Soak were performed. The efficacy of contact lens solutions was determined against keratitis-causing microbes, namely: Pseudomonas aeruginosa, Serratia marcescens, Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus, Fusarium solani and Acanthamoeba castellanii.
The results revealed that ReNu MultiPlus, DuraPlus and OptiFree Express were effective in killing bacterial and fungal pathogens as per manufacturer's minimum recommended disinfection time. Ultimate Plus was effective against F. solani and MRSA but ineffective against P. aeruginosa, S. marcescens and S. aureus. Of concern however, is that none of the locally formulated contact lens disinfecting solutions from Pakistan, i.e., Kontex Clean, Kontex Normal, Kontex Multisol extra(+) and Kontex Soak were effective against any of the keratitis-causing organisms tested. All eight contact lens disinfecting solutions were unable to destroy Acanthamoeba cysts.
Because such ineffective contact lens disinfection solutions present a major risk to public health, these findings are of great concern to the health officials and to the manufacturers of the contact lens disinfection solutions and effective solutions are needed, along with emphasis on proper hygiene for contact lens care and special guidelines for developing countries regarding the manufacture and storage of contact lens disinfecting solutions.
本研究旨在评估市售隐形眼镜消毒溶液的抗菌效果。
采用ISO 14729独立测试法对八种不同的隐形眼镜溶液进行杀菌、杀真菌和杀阿米巴检测，这八种溶液包括：ReNu MultiPlus、DuraPlus、Ultimate Plus、OptiFree Express、Kontex Clean、Kontex Normal、Kontex Multisol extra(+)、Kontex Soak。针对引起角膜炎的微生物，即铜绿假单胞菌、粘质沙雷氏菌、金黄色葡萄球菌、耐甲氧西林金黄色葡萄球菌、茄病镰刀菌和卡氏棘阿米巴，测定隐形眼镜溶液的功效。
结果显示，按照制造商推荐的最短消毒时间，ReNu MultiPlus、DuraPlus和OptiFree Express能有效杀灭细菌和真菌病原体。Ultimate Plus对茄病镰刀菌和耐甲氧西林金黄色葡萄球菌有效，但对铜绿假单胞菌、粘质沙雷氏菌和金黄色葡萄球菌无效。然而，令人担忧的是，巴基斯坦本地配制的隐形眼镜消毒溶液，即Kontex Clean、Kontex Normal、Kontex Multisol extra(+)和Kontex Soak，对所测试的任何引起角膜炎的生物体均无效。所有八种隐形眼镜消毒溶液均无法破坏棘阿米巴囊肿。
由于这种无效的隐形眼镜消毒溶液对公众健康构成重大风险，这些发现引起了卫生官员以及隐形眼镜消毒溶液制造商的高度关注。需要有效的溶液，同时强调隐形眼镜护理的正确卫生措施以及针对发展中国家隐形眼镜消毒溶液生产和储存的特殊指南。
