用于研究乙型肝炎病毒感染和药物开发的细胞和动物模型。
Cell and Animal Models for Studying Hepatitis B Virus Infection and Drug Development.
机构信息
The Pennsylvania State University College of Medicine, Hershey, Pennsylvania.
Graduate Institute of Clinical Medicine, National Taiwan University College of Medicine, Taipei, Taiwan.
出版信息
Gastroenterology. 2019 Jan;156(2):338-354. doi: 10.1053/j.gastro.2018.06.093. Epub 2018 Sep 19.
Abstract
Many cell culture and animal models have been used to study hepatitis B virus (HBV) replication and its effects in the liver; these have facilitated development of strategies to control and clear chronic HBV infection. We discuss the advantages and limitations of systems for studying HBV and developing antiviral agents, along with recent advances. New and improved model systems are needed. Cell culture systems should be convenient, support efficient HBV infection, and reproduce responses of hepatocytes in the human body. We also need animals that are fully permissive to HBV infection, convenient for study, and recapitulate human immune responses to HBV and effects in the liver. High-throughput screening technologies could facilitate drug development based on findings from cell and animal models.
摘要
许多细胞培养和动物模型被用于研究乙型肝炎病毒 (HBV) 的复制及其在肝脏中的作用；这些模型促进了控制和清除慢性 HBV 感染的策略的发展。我们讨论了用于研究 HBV 和开发抗病毒药物的系统的优缺点，以及最新进展。需要新的和改进的模型系统。细胞培养系统应该方便，支持有效的 HBV 感染，并复制人体肝细胞的反应。我们还需要对 HBV 感染完全允许、便于研究并重现人类对 HBV 的免疫反应和在肝脏中的作用的动物。高通量筛选技术可以根据细胞和动物模型的研究结果促进药物开发。
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