纳米顺铂-碳纳米管制剂对利什曼原虫的体外抗利什曼原虫活性。

Potent in vitro antileishmanial activity of a nanoformulation of cisplatin with carbon nanotubes against Leishmania major.

机构信息

Immunogenetics Research Center, Department of Medical Nanotechnology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.

Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran.

出版信息

J Glob Antimicrob Resist. 2019 Mar;16:11-16. doi: 10.1016/j.jgar.2018.09.004. Epub 2018 Sep 20.

DOI:10.1016/j.jgar.2018.09.004

PMID:30244039

Abstract

OBJECTIVES

The aim of this study was to evaluate the cytotoxicity and antileishmanial activity of cisplatin-bonded carbon nanotubes both against promastigotes and amastigotes of Leishmania major in vitro.

METHODS

Cisplatin-bonded single-walled carbon nanotubes (CP-SWCNT) and cisplatin-bonded multi-walled carbon nanotubes (CP-MWCNT) were considered as test compounds. In addition, SWCNT, MWCNT, free cisplatin and meglumine antimoniate (Glucantime) were considered as controls. The effect of each compound was evaluated both on promastigote and amastigote stages of L. major and the results were compared.

RESULTS

There was a statistically significant difference between the half-maximal inhibitory concentration (IC) of CP-SWCNT and each of the controls, including SWCNT, cisplatin and Glucantime (P<0.05). In addition, IC values of CP-MWCNT and each of the controls, including MWCNT, cisplatin and Glucantime, were significantly different both for promastigotes and amastigotes (P<0.05). However, the selectivity index (SI) of CP-SWCNT was <10 (5.23), indicating that this compound is not completely safe. Moreover, the SI values of CP-MWCNT (12.54) and Glucantime (16.28) were >10, indicating the selective effect of these two compounds on the parasite. Moreover, the IC of CP-MWCNT (0.11±0.09μM) for amastigotes was 41-fold lower than that of Glucantime (4.52±1.31μM), suggesting that a lower dose of CP-MWCNT in comparison with Glucantime is required to kill 50% of amastigotes.

CONCLUSIONS

According to the potent in vitro antileishmanial activity of CP-MWCNT at low concentration against L. major, we suggest that they are evaluated in an animal model.

摘要

目的

本研究旨在评估顺铂结合碳纳米管（CP-SWCNT 和 CP-MWCNT）对体外大蜡螟前鞭毛体和无鞭毛体的细胞毒性和抗利什曼原虫活性。

方法

CP-SWCNT 和 CP-MWCNT 被视为试验化合物。此外，SWCNT、MWCNT、游离顺铂和葡甲胺（Glucantime）被视为对照。评估了每种化合物对大蜡螟前鞭毛体和无鞭毛体阶段的影响，并进行了比较。

结果

CP-SWCNT 的半最大抑制浓度（IC）与 SWCNT、顺铂和 Glucantime 等对照组之间存在统计学差异（P<0.05）。此外，CP-MWCNT 与 MWCNT、顺铂和 Glucantime 等对照组的 IC 值在前鞭毛体和无鞭毛体阶段均有显著差异（P<0.05）。然而，CP-SWCNT 的选择性指数（SI）<10（5.23），表明该化合物不完全安全。此外，CP-MWCNT（12.54）和 Glucantime（16.28）的 SI 值>10，表明这两种化合物对寄生虫具有选择性作用。此外，CP-MWCNT 对无鞭毛体的 IC（0.11±0.09μM）比 Glucantime（4.52±1.31μM）低 41 倍，表明与 Glucantime 相比，CP-MWCNT 需要更低的剂量才能杀死 50%的无鞭毛体。