Birjand University of Medical Sciences, Department of Medical Microbiology, Infectious Disease Research Center, Birjand, İran
Mashhad University of Medical Sciences, Health Center, Mashhad, İran
Turkiye Parazitol Derg. 2021 Mar 2;45(1):1-4. doi: 10.4274/tpd.galenos.2020.6956.
Cutaneous leishmaniasis caused by () is an endemic disease in Iran. The current reference drugs, including Glucantime, possess high toxicity in addition to some side-effects. Therefore, there is a growing interest in exploring biomedical plants. The goal of the present study was to evaluate the anti-leishmanial activity and cytotoxicity of hydroalcoholic extracts from () over promastigote and amastigote forms.
This study was performed at the Iran Birjand University of Medical Sciences, during the year 2019. In this study, the hydroalcoholic extracts of the stems, leaves (LE) and fruits (FE) of were obtained. The anti-leishmanial activity was assessed against leptomonad promastigotes and intracellular amastigotes of . The cytotoxicity of these extracts was determined in murine macrophages.
The FE and LE of demonstrated a significant leishmanicidal effect against promastigotes with an IC50 of 0.9 mg/mL and 1.1 mg/mL, respectively. The FE showed the most anti-leishmanial activity and presented with the highest index of selectivity (SI=14.6) as an anti-leishmanial product. Infected macrophages treated using the FE showed a reduction in parasite burden by 97.3%.
The results of the present study demonstrated the leishmanicidal activity of on both promastigotes and intracellular amastigotes. There is a need for performing comprehensive studies on relevant animal models and to access the effects of active components of extract on the growth of L. major.
由（）引起的皮肤利什曼病是伊朗的地方性疾病。目前的参考药物，包括葡萄糖胺，除了一些副作用外，还具有很高的毒性。因此，人们越来越感兴趣于探索生物医学植物。本研究的目的是评估（）的水醇提取物对前鞭毛体和无鞭毛体形式的抗利什曼原虫活性和细胞毒性。
这项研究是在 2019 年于伊朗比尔詹德医科大学进行的。在这项研究中，获得了（）的茎、叶（LE）和果实（FE）的水醇提取物。抗利什曼原虫活性针对无鞭毛体前鞭毛体和（）的内阿米巴体进行评估。这些提取物的细胞毒性在鼠巨噬细胞中进行测定。
FE 和 LE 对（）的前鞭毛体表现出显著的杀利什曼原虫作用，IC50 分别为 0.9mg/mL 和 1.1mg/mL。FE 表现出最强的抗利什曼原虫活性，并表现出最高的选择性指数（SI=14.6）作为抗利什曼原虫产品。用 FE 处理感染的巨噬细胞可使寄生虫负荷减少 97.3%。
本研究结果表明，（）对前鞭毛体和内阿米巴体均具有杀利什曼原虫活性。需要在相关动物模型上进行全面的研究，并评估（）提取物的活性成分对 L. major 生长的影响。
