Lu Min, He Xiangwei
Life Sciences Institute and Innovation Center for Cell Signaling Network, Zhejiang University, Hangzhou, Zhejiang, 310058, China.
Curr Genet. 2019 Apr;65(2):381-386. doi: 10.1007/s00294-018-0886-9. Epub 2018 Sep 22.
In eukaryotes, the integrity of chromatin structure and organization is crucial to diverse key cellular processes from development to disease avoidance. To maintain the cell identity through mitotic cell generations, the genome (the genomic DNA sequence) as well as the epigenome (pertaining various forms of epigenetic information carriers, such as histone modifications, nucleosome positioning and the chromatin organization) is inherited with high fidelity. In comparison to the wealth of knowledge on genetic stability, we know much less on what may control the accuracy of epigenetic inheritance. In our recent work in the fission yeast Schizosaccharomyces pombe, by quantifying the epigenetic fidelity of CENP-A/Cnp1 or H3K9me2 nucleosome inheritance through cell divisions, we demonstrated that Ccp1, a homolog of histone chaperone Vps75 in budding yeast, participates in the modulation of centromeric nucleosomal epigenetic stability as well as proper heterochromatin organization. In this essay, we focus on discussing the uniquely high dynamicity of the subtelomeric heterochromatin regions and the complex mechanisms regulating epigenetic stability of centromeric chromatin.
在真核生物中，染色质结构和组织的完整性对于从发育到疾病预防等各种关键细胞过程至关重要。为了在有丝分裂细胞世代中维持细胞身份，基因组（基因组DNA序列）以及表观基因组（涉及各种形式的表观遗传信息载体，如组蛋白修饰、核小体定位和染色质组织）以高保真度遗传。与关于遗传稳定性的丰富知识相比，我们对控制表观遗传遗传准确性的因素了解得要少得多。在我们最近对裂殖酵母粟酒裂殖酵母的研究中，通过量化CENP-A/Cnp1或H3K9me2核小体在细胞分裂过程中的表观遗传保真度，我们证明了Ccp1，一种芽殖酵母中组蛋白伴侣Vps75的同源物，参与了着丝粒核小体表观遗传稳定性的调节以及适当的异染色质组织。在本文中，我们着重讨论亚端粒异染色质区域独特的高动态性以及调节着丝粒染色质表观遗传稳定性的复杂机制。
