巴西马托格罗索州自耕农农场猪中戊型肝炎病毒 3 基因型的检测。
Detection of hepatitis E virus genotype 3 in pigs from subsistence farms in the state of Mato Grosso, Brazil.
机构信息
Laboratory of Veterinary Pathology, Universidade Federal de Mato Grosso (UFMT), Av. Fernando Corrêa da Costa, 2367, Boa Esperança, CEP 78069-900, Cuiabá, Mato Grosso, Brazil.
Veterinary Virology Laboratory, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves, 9090, Agronomia, CEP 91540-000, Porto Alegre, Rio Grande do Sul, Brazil.
出版信息
Comp Immunol Microbiol Infect Dis. 2018 Jun;58:11-16. doi: 10.1016/j.cimid.2018.06.002. Epub 2018 Jun 13.
Hepatitis E is a zoonotic disease, recognized as an important global public health concern. In this study, molecular detection of the ORF1 and ORF2 genomic regions of the hepatitis E virus (HEV) was carried out in fecal and serum samples from pigs in subsistence farms of Mato Grosso, Brazil. Fragments of the ORF2 region were amplified in 8% (12/150) of fecal samples, with 53.3% (8/15) of farms having positive results. Of the 12 positive samples, fragments of the ORF1 region were amplified in 33.3% (4/12) of these. Molecular characterization confirmed the phylogenetic groupings as HEV subtypes 3d, 3 h, and 3i. The results revealed that meat from pigs that was originally meant for personal consumption is being traded in marketplaces in metropolitan Cuiabá, thereby creating a source of transmission to consumers in Mato Grosso. The environmental conditions must be taken into account when investigating the presence and transmission of HEV.
戊型肝炎是一种人畜共患疾病，被认为是一个重要的全球公共卫生关注点。在这项研究中，对巴西马托格罗索州维持生计农场的猪的粪便和血清样本进行了戊型肝炎病毒（HEV）的 ORF1 和 ORF2 基因组区域的分子检测。ORF2 区域的片段在 8%（12/150）的粪便样本中被扩增，其中 53.3%（8/15）的农场有阳性结果。在 12 个阳性样本中，ORF1 区域的片段在其中 33.3%（4/12）的样本中被扩增。分子特征确认了 HEV 亚型 3d、3h 和 3i 的分组。结果表明，原本供个人食用的猪肉正在大都市库亚巴的市场上交易，从而为马托格罗索州的消费者创造了传播源。在调查 HEV 的存在和传播时，必须考虑环境条件。
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