后院猪是巴西南部人畜共患戊型肝炎病毒的宿主。

Backyard pigs are a reservoir of zoonotic hepatitis E virus in southern Brazil.

作者信息

da Silva M S, Silveira S, Caron V S, Mósena A C S, Weber M N, Cibulski S P, Medeiros A A R, Silva G S, Corbellini L G, Klein R, Kreutz L C, Frandoloso R, Canal C W

机构信息

Laboratório de Virologia, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves 9090, CEP 91-540-000, Porto Alegre, Rio Grande do Sul, Brazil.

Laboratório de Epidemiologia, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil.

出版信息

Trans R Soc Trop Med Hyg. 2018 Jan 1;112(1):14-21. doi: 10.1093/trstmh/try017.

DOI:10.1093/trstmh/try017

PMID:29554365

Abstract

BACKGROUND

Hepatitis E virus (HEV) is the causative agent of acute hepatitis worldwide. There is no seroprevalence study in backyard farms, which are characterized by suboptimal hygienic conditions in Brazil. We aimed to determine the seroprevalence and genetic diversity of HEV in backyard pigs in Brazil.

METHODS

Swine serum samples collected in 2012 (n=731) and 2014 (n=713) were analysed. The presence of anti-HEV immunoglobulin G in pig serum was evaluated by indirect enzyme-linked immunosorbent assay. Reverse transcription polymerase chain reaction was performed and phylogenetic analyses were carried out based on the partial ORF1 and ORF2 coding regions.

RESULTS

Anti-HEV antibodies were detected in 77.6% (567/731; 95% confidence interval [CI] 74.5 to 90.6%) of serum samples in 2012 and 65.5% (467/713; 95% CI 62.0 to 69.0%) in 2014. The herd seroprevalence was 91.7% (187/204; 95% CI 91% to 99%) in 2012 and 83.7% (164/196; 95% CI 78% to 89%) in 2014. Further, HEV RNA was detected in 0.8% (6/713) of samples from 2014. Phylogenetic analysis showed three different genotype 3 subtypes with high similarity to human HEV strains.

CONCLUSIONS

This study showed that backyard pigs are a reservoir of HEV and alerts us to the need to control infection and spillover from backyard farms.

GENBANK ACCESSION NUMBERS

MF438128-MF438135.

摘要

背景

戊型肝炎病毒（HEV）是全球急性肝炎的病原体。在巴西，以卫生条件欠佳为特征的家庭养殖场尚未开展血清流行率研究。我们旨在确定巴西家庭养殖猪中HEV的血清流行率和基因多样性。

方法

对2012年（n = 731）和2014年（n = 713）采集的猪血清样本进行分析。通过间接酶联免疫吸附试验评估猪血清中抗HEV免疫球蛋白G的存在情况。进行逆转录聚合酶链反应，并基于部分ORF1和ORF2编码区域开展系统发育分析。

结果

2012年77.6%（567/731；95%置信区间[CI] 74.5至90.6%）的血清样本检测到抗HEV抗体，2014年为65.5%（467/713；95% CI 62.0至69.0%）。2012年猪群血清流行率为91.7%（187/204；95% CI 91%至99%），2014年为83.7%（164/196；95% CI 78%至89%）。此外，2014年0.8%（6/713）的样本检测到HEV RNA。系统发育分析显示三种不同的基因型3亚型，与人类HEV毒株高度相似。