季也蒙念珠菌中的细胞色素P-450和烷烃羟化酶活性
Cytochrome P-450 and alkane hydroxylase activity in Candida guilliermondii.
作者信息
Schunck W H, Riege P, Blasig R, Honeck H, Müller H G
出版信息
Acta Biol Med Ger. 1978;37(1):K3-K7.
PMID:30246
Abstract
In the investigated Candida guilliermondii strain after growth on n-alkanes as the only carbon and energy source 5--10 nMol cytochrome P-450 per g cells (wet weight) could be detected. Cytochrome P-450 and alkane hydroxylase activity was found in the 100 000 xg pellet. Cofactor studies and inhibition experiments revealed the existence of a NADPH-dependent cytochrome P-450 alkane hydroxylase system.
摘要
在所研究的季也蒙毕赤酵母菌株中，以正构烷烃作为唯一碳源和能源生长后，每克细胞（湿重）可检测到5-10纳摩尔细胞色素P-450。细胞色素P-450和烷烃羟化酶活性存在于100000×g离心所得的沉淀中。辅因子研究和抑制实验表明存在一种依赖于NADPH的细胞色素P-450烷烃羟化酶系统。
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