大鼠肝细胞膜中中性鞘磷脂酶的磷脂依赖性

Phospholipid dependence of the neutral sphingomyelinase in rat liver plasma membranes.

作者信息

Petkova D H, Momchilova A B, Koumanov K S

出版信息

Biochimie. 1986 Oct-Nov;68(10-11):1195-200. doi: 10.1016/s0300-9084(86)80064-5.

Abstract

Investigations have been carried out on the influence of the phospholipid composition of rat liver plasma membranes and of their physico-chemical properties on the activity of membrane-bound neutral sphingomyelinase. The membrane phospholipid composition was modified by the incorporation of different phospholipids into the membrane bilayer by means of lipid transfer proteins, n-butanol delipidation or exogenous sphingomyelinase (Staphylococcus aureus) treatment. The results indicate that the activity of neutral sphingomyelinase in liver plasma membranes depends upon phosphatidyl choline presence in the membrane bilayer and not upon membrane fluidity.

摘要

已经对大鼠肝细胞膜的磷脂组成及其物理化学性质对膜结合中性鞘磷脂酶活性的影响进行了研究。通过脂质转移蛋白将不同的磷脂掺入膜双层、正丁醇脱脂或用外源性鞘磷脂酶（金黄色葡萄球菌）处理来改变膜磷脂组成。结果表明，肝细胞膜中中性鞘磷脂酶的活性取决于膜双层中磷脂酰胆碱的存在，而不取决于膜流动性。