Qin Cheng, Bai Yijiang, Zeng Zhen, Wang Liao, Luo Zhiwen, Wang Shunqi, Zou Suqi
Institute of Life Science, Nanchang University; Queen Mary School, Medical Department, Nanchang University.
Institute of Life Science, Nanchang University; School of Life Science, Nanchang University.
J Vis Exp. 2018 Sep 5(139):58288. doi: 10.3791/58288.
Sectioning of the paraffin-embedded tissue is widely used in histology and pathology. However, it is tedious. To improve this method, several commercial companies have devised complex section transfer systems using fluid water. To simplify this technology, we created a simple method using homemade equipment that combines cutting and floating within a simple thermostatic chamber; therefore, the sections automatically enter the water bath on the water surface. The hippocampus from adult mouse brains, adult mouse kidneys, embryonic mouse brains, and adult zebrafish eyes were cut using both conventional paraffin sectioning and the presented method for comparison. Statistical analysis shows that our improved method saved time and produced higher quality sections. In addition, paraffin sectioning of a whole specimen in a short time is easy for junior operators.
石蜡包埋组织切片在组织学和病理学中被广泛应用。然而，这一过程很繁琐。为改进该方法，几家商业公司设计了使用流动水的复杂切片转移系统。为简化这项技术，我们创建了一种使用自制设备的简单方法，该设备在一个简单的恒温箱内将切割和漂浮步骤结合起来；因此，切片会自动进入水面上的水浴中。使用传统石蜡切片法和本文介绍的方法对成年小鼠大脑的海马体、成年小鼠肾脏、胚胎小鼠大脑以及成年斑马鱼眼睛进行切片，以便进行比较。统计分析表明，我们改进后的方法节省了时间，并且切片质量更高。此外，对于初级操作人员来说，在短时间内对整个标本进行石蜡切片很容易。
