Cavallari Eleonora, Carrera Carla, Aime Silvio, Reineri Francesca
Dept. Molecular Biotechnology and Health Sciences, University of Torino, Via Nizza 52, Torino, Italy.
Chemphyschem. 2019 Jan 21;20(2):318-325. doi: 10.1002/cphc.201800652. Epub 2018 Oct 24.
The kinetics of metabolic processes can be assessed, in real time by means of MR hyperpolarized (HP) metabolites. [1- C]pyruvate, hyperpolarized by means of d-DNP, is, by far, the substrate most widely applied to the investigation of several pathologies characterized by deregulated glycolytic metabolic networks, including cancer. Hyperpolarization of [1- C]pyruvate by means of the cost effective, fast and easy to handle PHIP-SAH (para-hydrogen induced polarization-side arm hydrogenation) method opens-up a pathway for the application of HP metabolites to a wide range of cancer-related studies. Herein, we report the first application of PHIP-SAH hyperpolarized [1- C]pyruvate in the investigation of upregulated glycolysis in two murine breast cancer cell lines (168FARN and 4T1). The results obtained using HP pyruvate have been validated with a conventional biochemical assay and are coherent with previously-reported lactate dehydrogenase activity measured in those cells.
代谢过程的动力学可以通过磁共振超极化（HP）代谢物进行实时评估。通过d-DNP超极化的[1- C]丙酮酸是迄今为止最广泛应用于研究几种以糖酵解代谢网络失调为特征的疾病（包括癌症）的底物。通过具有成本效益、快速且易于操作的PHIP-SAH（对氢诱导极化-侧链氢化）方法对[1- C]丙酮酸进行超极化，为将HP代谢物应用于广泛的癌症相关研究开辟了一条途径。在此，我们报告了PHIP-SAH超极化[1- C]丙酮酸在两种小鼠乳腺癌细胞系（168FARN和4T1）糖酵解上调研究中的首次应用。使用HP丙酮酸获得的结果已通过传统生化测定法进行验证，并且与先前报道的在这些细胞中测量的乳酸脱氢酶活性一致。
Zotero 插件