Cavallari Eleonora, Carrera Carla, Aime Silvio, Reineri Francesca
Department of Molecular Biotechnology and Health Sciences, University of Torino, via Nizza 52, Torino, Italy.
Istituto Bioimmagini e Biostrutture del CNR, Sezione di Torino c/o Center of Molecular Imaging, Via Nizza 52, Torino, Italy.
Chemistry. 2017 Jan 23;23(5):1200-1204. doi: 10.1002/chem.201605329. Epub 2016 Dec 19.
Hyperpolarization of the C magnetic resonance signal of l-[1- C]lactate has been obtained using the chemically based, cost-effective method called parahydrogen-induced polarization by means of side-arm hydrogenation (PHIP-SAH). Two ester derivatives of lactate were tested and the factors that determine the polarization level on the product have been investigated in detail. The metabolic conversion of hyperpolarized l-[1- C]lactate into pyruvate has been observed in vitro using lactate dehydrogenase (LDH) and in a cells lysate. From the acquisition of a series of C NMR spectra, the metabolic build-up of the [1- C]pyruvate signal has been observed. These studies demonstrate that, even if the experimental set-up used for these PHIP-SAH hyperpolarization studies is still far from optimal, the attained polarization level is already sufficient to carry out in vitro metabolic studies.
通过基于化学的、具有成本效益的侧臂氢化仲氢诱导极化法（PHIP-SAH），已实现了l-[1-¹³C]乳酸的¹³C磁共振信号的超极化。测试了乳酸的两种酯衍生物，并详细研究了决定产物极化水平的因素。使用乳酸脱氢酶（LDH）在体外以及在细胞裂解物中观察到了超极化的l-[1-¹³C]乳酸向丙酮酸的代谢转化。通过采集一系列¹³C NMR光谱，观察到了[1-¹³C]丙酮酸信号的代谢积累。这些研究表明，即使用于这些PHIP-SAH超极化研究的实验装置仍远非最佳，但所达到的极化水平已足以进行体外代谢研究。
