Amombo Erick, Li Xiaoning, Wang Guangyang, An Shao, Wang Wei, Fu Jinmin
Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture and Wuhan Botanical Garden, Chinese Academy of Sciences Wuhan, Wuhan 430074, China.
The University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing 100049, China.
Genes (Basel). 2018 Sep 21;9(10):466. doi: 10.3390/genes9100466.
Soil salinity is a serious threat to plant growth and crop productivity. Tall fescue utilization in saline areas is limited by its inferior salt tolerance. Thus, a transcriptome study is a prerequisite for future research aimed at providing deeper insights into the molecular mechanisms of tall fescue salt tolerance as well as molecular breeding. Recent advances in sequencing technology offer a platform to achieve this. Here, Illumina RNA sequencing of tall fescue leaves generated a total of 144,339 raw reads. After de novo assembly, unigenes with a total length of 129,749,938 base pairs were obtained. For functional annotations, the unigenes were aligned to various databases. Further structural analyses revealed 79,352 coding DNA sequences and 13,003 microsatellites distributed across 11,277 unigenes as well as single nucleotide polymorphisms. In total, 1862 unigenes were predicted to encode for 2120 transcription factors among which most were key salt-responsive. We determined differential gene expression and distribution per sample and most genes related to salt tolerance and photosynthesis were upregulated in 48 h vs. 24 h salt treatment. Protein interaction analysis revealed a high interaction of chaperonins and Rubisco proteins in 48 h vs. 24 h salt treatment. The gene expressions were finally validated using quantitative polymerase chain reaction (qPCR), which was coherent with sequencing results.
土壤盐渍化是对植物生长和作物生产力的严重威胁。高羊茅在盐碱地的利用因其较差的耐盐性而受到限制。因此，转录组研究是未来旨在深入了解高羊茅耐盐分子机制以及分子育种研究的前提条件。测序技术的最新进展提供了实现这一目标的平台。在此，对高羊茅叶片进行Illumina RNA测序共产生了144,339条原始读数。经过从头组装，获得了总长度为129,749,938个碱基对的单基因。为了进行功能注释，将单基因与各种数据库进行比对。进一步的结构分析揭示了79,352个编码DNA序列和13,003个微卫星，分布在11,277个单基因以及单核苷酸多态性中。总共预测有1862个单基因编码2120个转录因子，其中大多数是关键的盐响应因子。我们确定了每个样本的差异基因表达和分布，与24小时盐处理相比，大多数与耐盐性和光合作用相关的基因在48小时盐处理中上调。蛋白质相互作用分析显示，与24小时盐处理相比，伴侣蛋白和Rubisco蛋白在48小时盐处理中有高度相互作用。最终使用定量聚合酶链反应（qPCR）验证了基因表达，其与测序结果一致。
