Kumar Manu, Choi Juyoung, An Gynheung, Kim Seong-Ryong
Department of Life Science, Sogang University Seoul, South Korea.
Department of Plant Molecular Systems Biotechnology, Kyung Hee University Yongin, South Korea.
Front Plant Sci. 2017 Mar 10;8:316. doi: 10.3389/fpls.2017.00316. eCollection 2017.
Salt stress can severely reduce crop yields. To understand how rice () plants respond to this environmental challenge, we investigated the genes involved in conferring salt tolerance by screening T-DNA tagging lines and identified (alt olerance ctivation -ominant). In that line, expression of was enhanced by approximately eightfold when compared with the non-transformed wild type (WT). This gene was highly expressed in the callus, roots, and panicles. To confirm its role in stress tolerance, we generated transgenic rice that over-expresses under a maize promoter. The Ox plants were salt-tolerant at the vegetative stage, based on our calculations of chlorophyll fluorescence (Fv/Fm), fresh and dry weights, chlorophyll concentrations, and survival rates. Under normal paddy field conditions, the Ox plants were somewhat shorter than the WT control but had improved agronomic traits such as higher total grain yield. They were also more tolerant to osmotic stress and hypersensitive to abscisic acid. Based on all of these results, we suggest that has important roles in determining yields as well as in conferring tolerance to salt stresses.
盐胁迫会严重降低作物产量。为了解水稻植株如何应对这一环境挑战，我们通过筛选T-DNA标签系研究了参与赋予耐盐性的基因，并鉴定出了（耐盐激活显性基因）。在该株系中，与未转化的野生型（WT）相比，的表达增强了约八倍。该基因在愈伤组织、根和穗中高度表达。为了证实其在胁迫耐受性中的作用，我们培育了在玉米启动子下过表达的转基因水稻。根据我们对叶绿素荧光（Fv/Fm）、鲜重和干重、叶绿素浓度及存活率的计算，过表达植株在营养生长阶段具有耐盐性。在正常稻田条件下，过表达植株比野生型对照略矮，但具有诸如更高的总粒重等改良的农艺性状。它们对渗透胁迫也更具耐受性，对脱落酸超敏感。基于所有这些结果，我们认为在决定产量以及赋予盐胁迫耐受性方面具有重要作用。
