Netto A O, Gelaleti R B, Corvino S B, Serrano R G, Hernández S C, Volpato G T, Rudge M V C, Braz M G, Damasceno D C
Postgraduate Course on Gynecology, Obstetrics and Mastology, Laboratory of Experimental Research on Gynecology and Obstetrics, Botucatu Medical School, São Paulo State University - UNESP, Botucatu, São Paulo State, Brazil.
Biochemical Department, Havana Medical Science University, ICBP Victoria de Girón, Havana, Cuba.
Mutat Res Genet Toxicol Environ Mutagen. 2018 Nov;835:11-15. doi: 10.1016/j.mrgentox.2018.08.006. Epub 2018 Aug 18.
Fetal impairment caused by a deleterious intrauterine environment may have long-term consequences, such as oxidative stress and genetic damage. Rats born as small-for-gestational-age (SPA) were submitted to exercise (swimming) before and during pregnancy. The animals exhibited glucose intolerance, reduced general adiposity, and increased maternal and offspring organ weight, showing the benefit of exercise for these rats. We hypothesised that regular exercise in SPA during gestation could prevent DNA damage in these animals and in their offspring, contributing to altered fetal programming of metabolism in the offspring. Severe diabetes was induced by streptozotocin treatment, to obtain SPA newborns. At adulthood, pregnant SPA rats were randomly distributed into two groups: exercised (SPAex - submitted to swimming program) or not-exercised (SPA - sedentary rats). Post-partum, blood was collected for analysis of DNA damage (comet assay) and oxidative stress. SPAex rats presented lower DNA damage levels, decreased lipid peroxidation, and a lower rate of newborns classified as large-for-pregnancy-age. DNA damage was also lower in SPAex newborns. We conclude that swimming applied to SPA pregnant rats contributes to decreased DNA damage and lipid peroxidation in the dams, and decreased DNA damage and macrosomia in their offspring.
有害的子宫内环境导致的胎儿损伤可能会产生长期后果，如氧化应激和基因损伤。出生时为小于胎龄儿（SGA）的大鼠在怀孕前和怀孕期间进行运动（游泳）。这些动物表现出葡萄糖不耐受、总体脂肪减少以及母体和后代器官重量增加，表明运动对这些大鼠有益。我们假设，孕期SGA大鼠进行规律运动可预防这些动物及其后代的DNA损伤，有助于改变后代胎儿的代谢编程。通过链脲佐菌素治疗诱导严重糖尿病，以获得SGA新生大鼠。成年后，怀孕的SGA大鼠被随机分为两组：运动组（SGAex - 进行游泳项目）或非运动组（SGA - 久坐不动的大鼠）。产后，采集血液用于分析DNA损伤（彗星试验）和氧化应激。SGAex大鼠的DNA损伤水平较低，脂质过氧化减少，且被归类为大于孕龄儿的新生儿比例较低。SGAex新生大鼠的DNA损伤也较低。我们得出结论，对怀孕的SGA大鼠进行游泳运动有助于降低母鼠的DNA损伤和脂质过氧化，并降低其后代的DNA损伤和巨大儿发生率。
