• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

U 小核 RNA 3' 末端的形成至少需要三个序列元件。

Formation of the 3' end on U snRNAs requires at least three sequence elements.

作者信息

Ciliberto G, Dathan N, Frank R, Philipson L, Mattaj I W

出版信息

EMBO J. 1986 Nov;5(11):2931-7. doi: 10.1002/j.1460-2075.1986.tb04589.x.

DOI:10.1002/j.1460-2075.1986.tb04589.x
PMID:3024969
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1167244/
Abstract

The structural requirements for 3' end formation on the Xenopus laevis U1B snRNA gene have been studied. Three sequence elements are shown to be required. The first is a conserved sequence element found immediately 3' of all vertebrate U snRNA genes studied so far. The second is a gene internal sequence potentially capable of forming a stem-loop structure close to the 3' end of the RNA. The third element lies upstream of these, and may be part of the gene promoter. Experiments designed to investigate the mechanism of 3' end formation on primary U1B snRNA transcripts failed to find evidence for a processing event.

摘要

对非洲爪蟾U1B小核RNA（snRNA）基因3'端形成的结构要求进行了研究。结果表明需要三个序列元件。第一个是到目前为止在所有已研究的脊椎动物U snRNA基因3'端紧邻位置发现的保守序列元件。第二个是基因内部的一个序列，它有可能在RNA的3'端附近形成一个茎环结构。第三个元件位于这些元件的上游，可能是基因启动子的一部分。旨在研究初级U1B snRNA转录本3'端形成机制的实验未能找到加工事件的证据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b788/1167244/f5c60a81f477/emboj00174-0195-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b788/1167244/9c6c86c3ae92/emboj00174-0192-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b788/1167244/0d5af0a6a046/emboj00174-0193-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b788/1167244/6c5fb3d503fe/emboj00174-0194-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b788/1167244/874fb9f6a97b/emboj00174-0194-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b788/1167244/f5c60a81f477/emboj00174-0195-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b788/1167244/9c6c86c3ae92/emboj00174-0192-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b788/1167244/0d5af0a6a046/emboj00174-0193-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b788/1167244/6c5fb3d503fe/emboj00174-0194-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b788/1167244/874fb9f6a97b/emboj00174-0194-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b788/1167244/f5c60a81f477/emboj00174-0195-a.jpg

相似文献

1
Formation of the 3' end on U snRNAs requires at least three sequence elements.U 小核 RNA 3' 末端的形成至少需要三个序列元件。
EMBO J. 1986 Nov;5(11):2931-7. doi: 10.1002/j.1460-2075.1986.tb04589.x.
2
Properties of a U1 RNA enhancer-like sequence.一种U1 RNA增强子样序列的特性。
Nucleic Acids Res. 1987 Mar 25;15(6):2403-16. doi: 10.1093/nar/15.6.2403.
3
Formation of the 3' end of sea urchin U1 small nuclear RNA occurs independently of the conserved 3' box and on transcripts initiated from a histone promoter.海胆U1小核RNA 3'末端的形成独立于保守的3'框，且发生在由组蛋白启动子起始的转录本上。
Mol Cell Biol. 1992 Sep;12(9):4132-41. doi: 10.1128/mcb.12.9.4132-4141.1992.
4
Functional characterization of X. laevis U5 snRNA genes.非洲爪蟾U5小核RNA基因的功能特性
EMBO J. 1987 Oct;6(10):3071-8. doi: 10.1002/j.1460-2075.1987.tb02614.x.
5
Transcription of satellite 2 DNA from the newt is driven by a snRNA type of promoter.
Nucleic Acids Res. 1994 Nov 11;22(22):4697-704. doi: 10.1093/nar/22.22.4697.
6
Functional, developmentally expressed genes for mouse U1a and U1b snRNAs contain both conserved and non-conserved transcription signals.小鼠U1a和U1b小核RNA的功能性、发育表达基因包含保守和非保守的转录信号。
Nucleic Acids Res. 1986 Dec 22;14(24):9811-25. doi: 10.1093/nar/14.24.9811.
7
Activity of chimeric U small nuclear RNA (snRNA)/mRNA genes in transfected protoplasts of Nicotiana plumbaginifolia: U snRNA 3'-end formation and transcription initiation can occur independently in plants.嵌合U小核RNA（snRNA）/mRNA基因在蓝烟草转染原生质体中的活性：U snRNA 3'末端形成和转录起始在植物中可独立发生。
Mol Cell Biol. 1993 Oct;13(10):6403-15. doi: 10.1128/mcb.13.10.6403-6415.1993.
8
The trimethylguanosine cap structure of U1 snRNA is a component of a bipartite nuclear targeting signal.U1小核核糖核酸（snRNA）的三甲基鸟苷帽结构是双组分核定位信号的一个组成部分。
Cell. 1990 Aug 10;62(3):569-77. doi: 10.1016/0092-8674(90)90021-6.
9
Initiation and termination of human U1 RNA transcription requires the concerted action of multiple flanking elements.人类U1 RNA转录的起始和终止需要多个侧翼元件的协同作用。
Nucleic Acids Res. 1989 Nov 25;17(22):9305-18. doi: 10.1093/nar/17.22.9305.
10
Increasing the distance between the snRNA promoter and the 3' box decreases the efficiency of snRNA 3'-end formation.增加小核仁RNA（snRNA）启动子与3'框之间的距离会降低snRNA 3'末端形成的效率。
Nucleic Acids Res. 1996 Nov 15;24(22):4525-34. doi: 10.1093/nar/24.22.4525.

引用本文的文献

1
Structure of the catalytic core of the Integrator complex.整合酶复合物催化核心的结构。
Mol Cell. 2021 Mar 18;81(6):1246-1259.e8. doi: 10.1016/j.molcel.2021.01.005. Epub 2021 Feb 5.
2
Non-mRNA 3' end formation: how the other half lives.非 mRNA 3' 端形成：另一半的生命。
Wiley Interdiscip Rev RNA. 2013 Sep-Oct;4(5):491-506. doi: 10.1002/wrna.1174. Epub 2013 Jun 10.
3
U1-like snRNAs lacking complementarity to canonical 5' splice sites.与典型5'剪接位点缺乏互补性的U1样小核RNA

本文引用的文献

1
Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.在哺乳动物细胞中表达氯霉素乙酰转移酶的重组基因组。
Mol Cell Biol. 1982 Sep;2(9):1044-51. doi: 10.1128/mcb.2.9.1044-1051.1982.
2
Formation of the 3' end of histone mRNA by post-transcriptional processing.通过转录后加工形成组蛋白mRNA的3'末端。
Nature. 1984;308(5955):203-6. doi: 10.1038/308203a0.
3
3' editing of mRNAs: sequence requirements and involvement of a 60-nucleotide RNA in maturation of histone mRNA precursors.信使核糖核酸的3'端编辑：序列要求及一段60个核苷酸的核糖核酸在组蛋白信使核糖核酸前体成熟过程中的作用
RNA. 2006 Sep;12(9):1603-11. doi: 10.1261/rna.26506. Epub 2006 Jul 7.
4
The 3' ends of human pre-snRNAs are produced by RNA polymerase II CTD-dependent RNA processing.人类前体小核RNA的3'末端是由RNA聚合酶II CTD依赖性RNA加工产生的。
EMBO J. 2003 Sep 1;22(17):4544-54. doi: 10.1093/emboj/cdg430.
5
The 3' end formation in small RNAs.小RNA中3'端的形成
Gene Expr. 2002;10(1-2):59-78.
6
Pac1p, an RNase III homolog, is required for formation of the 3' end of U2 snRNA in Schizosaccharomyces pombe.Pac1p是一种核糖核酸酶III同源物，在粟酒裂殖酵母中，它是U2小核RNA 3'末端形成所必需的。
RNA. 1999 Aug;5(8):1083-98. doi: 10.1017/s1355838299990726.
7
Transcription of the human U2 snRNA genes continues beyond the 3' box in vivo.在体内，人类U2小核RNA基因的转录会延续至3'框之外。
EMBO J. 1999 May 17;18(10):2867-77. doi: 10.1093/emboj/18.10.2867.
8
Alternative 3'-end processing of U5 snRNA by RNase III.核糖核酸酶III对U5小核仁RNA进行替代性3'端加工。
Genes Dev. 1997 Oct 15;11(20):2741-51. doi: 10.1101/gad.11.20.2741.
9
Characterization of the mouse beta maj globin transcription termination region: a spacing sequence is required between the poly(A) signal sequence and multiple downstream termination elements.小鼠β珠蛋白基因主要转录本转录终止区域的特征：在聚腺苷酸化信号序列与多个下游终止元件之间需要一个间隔序列。
Mol Cell Biol. 1993 Jan;13(1):578-87. doi: 10.1128/mcb.13.1.578-587.1993.
10
Activity of chimeric U small nuclear RNA (snRNA)/mRNA genes in transfected protoplasts of Nicotiana plumbaginifolia: U snRNA 3'-end formation and transcription initiation can occur independently in plants.嵌合U小核RNA（snRNA）/mRNA基因在蓝烟草转染原生质体中的活性：U snRNA 3'末端形成和转录起始在植物中可独立发生。
Mol Cell Biol. 1993 Oct;13(10):6403-15. doi: 10.1128/mcb.13.10.6403-6415.1993.
Proc Natl Acad Sci U S A. 1984 Feb;81(4):1057-61. doi: 10.1073/pnas.81.4.1057.
4
Human beta-globin pre-mRNA synthesized in vitro is accurately spliced in Xenopus oocyte nuclei.在体外合成的人β-珠蛋白前体信使核糖核酸（pre-mRNA）在非洲爪蟾卵母细胞核中能被精确剪接。
Cell. 1983 Mar;32(3):681-94. doi: 10.1016/0092-8674(83)90054-5.
5
The 3' end of drosophila histone H3 mRNA is produced by a processing activity in vitro.果蝇组蛋白H3 mRNA的3'端是在体外通过一种加工活性产生的。
Cell. 1984 Sep;38(2):423-9. doi: 10.1016/0092-8674(84)90497-5.
6
New M13 vectors for cloning.用于克隆的新型M13载体。
Methods Enzymol. 1983;101:20-78. doi: 10.1016/0076-6879(83)01005-8.
7
Transcription of the beta-like globin genes and pseudogenes of the goat in a cell-free system.山羊β样珠蛋白基因和假基因在无细胞系统中的转录
Nucleic Acids Res. 1981 Sep 11;9(17):4339-54. doi: 10.1093/nar/9.17.4339.
8
The 5' terminus of the RNA moiety of U1 small nuclear ribonucleoprotein particles is required for the splicing of messenger RNA precursors.U1小核核糖核蛋白颗粒的RNA部分的5'末端是信使RNA前体剪接所必需的。
Cell. 1984 Aug;38(1):299-307. doi: 10.1016/0092-8674(84)90551-8.
9
Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors.克隆至M13载体的DNA片段的寡核苷酸定向诱变
Methods Enzymol. 1983;100:468-500. doi: 10.1016/0076-6879(83)00074-9.
10
Synthesis of human U1 RNA. II. Identification of two regions of the promoter essential for transcription initiation at position +1.人U1 RNA的合成。II. 对转录起始于+1位置所必需的启动子两个区域的鉴定。
J Biol Chem. 1984 Jul 10;259(13):8345-52.