来自于古山龙树皮的孕烷糖苷及其细胞毒性。

Pregnane glycosides from the bark of Marsdenia cundurango and their cytotoxic activity.

机构信息

Department of Medicinal Pharmacognosy, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo, 192-0392, Japan.

出版信息

J Nat Med. 2019 Jan;73(1):93-103. doi: 10.1007/s11418-018-1248-0. Epub 2018 Sep 24.

DOI:10.1007/s11418-018-1248-0

PMID:30251034

Abstract

Seven new pregnane glycosides (1-7) and eight known compounds (8-15) were isolated from the bark of Marsdenia cundurango (Asclepiadaceae). The structures of 1-7 were determined by spectroscopic analysis, including two-dimension NMR spectroscopy, chemical transformations, and chromatographic analysis of the hydrolyzed products. The isolated compounds 1-15 were evaluated for their cytotoxic activity against HL-60 human leukemia cells, A549 human lung adenocarcinoma cells, and TIG-3 normal human lung cells, including apoptosis-inducing activity of a representative pregnane glycoside in HL-60 cells.

摘要

从云南马兜铃（马兜铃科）的树皮中分离得到了 7 个新的孕甾烷糖苷（1-7）和 8 个已知化合物（8-15）。通过光谱分析，包括二维 NMR 光谱、化学转化和水解产物的色谱分析，确定了 1-7 的结构。对分离得到的化合物 1-15 进行了人白血病 HL-60 细胞、人肺腺癌细胞 A549 和正常肺细胞 TIG-3 的细胞毒性活性评价，包括对 HL-60 细胞中代表性孕甾烷糖苷的诱导凋亡活性评价。