Shao Meiqi, Chen Yan, Peng Qing, Liu Pengfu, Wu Jiequn, Chu Xiaohe
Zhejiang University of Technology, Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Hangzhou 310014, China.
Zhejiang Yangtze Delta Region Pharmaceutical Technology Research Park, Deqing 313200, China.
Se Pu. 2018 Aug 8;36(8):817-821. doi: 10.3724/SP.J.1123.2018.03026.
A method was developed for the determination of the content of aminobutanol by high performance liquid chromatography (HPLC) based on charge transfer reaction. Under the condition of a borax-boric acid buffer solution of pH 8.4, aminobutanol and tetra-chloro-benzoquinone reacted at 60℃ for 60 min, and were analyzed by an HPLC-ultraviolet detector. The charge-transfer complex was separated on an Agilent Extend C18 column (250 mm×4.6 mm, 5 μm) with 0.001% (v/v) triethylamine and methanol as the mobile phases for gradient elution at a flow rate of 1 mL/min. The limit of quantification of aminobutanol was 0.01 g/L, the linear range was 0.1-0.6 g/L, and the correlation coefficient () was 0.9994. The spiked recoveries of the method were 98.3%-103.6% with relative standard deviations (RSDs) of 0.9%-1.6%. The method is simple and quick, and suitable for the rapid detection of aminobutanol.
