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大肠杆菌K-12中aroP基因的转录调控：操纵子突变体分析

Transcription control of the aroP gene in Escherichia coli K-12: analysis of operator mutants.

作者信息

Chye M L, Pittard J

出版信息

J Bacteriol. 1987 Jan;169(1):386-93. doi: 10.1128/jb.169.1.386-393.1987.

DOI:10.1128/jb.169.1.386-393.1987
PMID:3025182
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC211779/
Abstract

The nucleotide sequence of the region containing the promoter-operator for the aroP gene was determined. The start site of aroP transcription was identified by using S1 nuclease mapping and primer extension techniques. Examination of the nucleotide sequence revealed the presence of two "TYR R" boxes which are similar to those identified in the regulatory regions of other genes in the tyrR regulon. Bisulfite-induced aroP operator-constitutive mutants were analyzed, and the base-pair changes responsible for alterations in aroP regulation were located within these boxes.

摘要

确定了包含aroP基因启动子-操纵子区域的核苷酸序列。通过使用S1核酸酶图谱分析和引物延伸技术确定了aroP转录的起始位点。对核苷酸序列的检查揭示了存在两个“TYR R”框，它们与tyrR调节子中其他基因的调节区域中鉴定出的框相似。分析了亚硫酸氢盐诱导的aroP操纵子组成型突变体，负责aroP调节改变的碱基对变化位于这些框内。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/729c/211779/23396a350f81/jbacter00191-0408-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/729c/211779/1e3403bbc95c/jbacter00191-0408-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/729c/211779/23396a350f81/jbacter00191-0408-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/729c/211779/1e3403bbc95c/jbacter00191-0408-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/729c/211779/23396a350f81/jbacter00191-0408-b.jpg

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本文引用的文献

1
[The biosynthesis of beta-galactosidase (lactase) in Escherichia coli; the specificity of induction].[大肠杆菌中β-半乳糖苷酶（乳糖酶）的生物合成；诱导的特异性]
Biochim Biophys Acta. 1951 Nov;7(4):585-99. doi: 10.1016/0006-3002(51)90072-8.
2
UPTAKE OF AMINO ACIDS BY SALMONELLA TYPHIMURIUM.鼠伤寒沙门氏菌对氨基酸的摄取
Arch Biochem Biophys. 1964 Jan;104:1-18. doi: 10.1016/s0003-9861(64)80028-x.
3
Local mutagenesis within deletion loops of DNA heteroduplexes.DNA异源双链体缺失环内的局部诱变
鉴定LIV-I/LS系统为大肠杆菌K-12中的第三种苯丙氨酸转运体。
J Bacteriol. 2004 Jan;186(2):343-50. doi: 10.1128/JB.186.2.343-350.2004.
4
Mechanism of repression of the aroP P2 promoter by the TyrR protein of Escherichia coli.大肠杆菌TyrR蛋白对aroP P2启动子的抑制机制。
J Bacteriol. 1999 Oct;181(20):6411-8. doi: 10.1128/JB.181.20.6411-6418.1999.
5
Demonstration that the TyrR protein and RNA polymerase complex formed at the divergent P3 promoter inhibits binding of RNA polymerase to the major promoter, P1, of the aroP gene of Escherichia coli.证明在发散型P3启动子处形成的TyrR蛋白与RNA聚合酶复合物会抑制RNA聚合酶与大肠杆菌aroP基因的主要启动子P1的结合。
J Bacteriol. 1998 Oct;180(20):5466-72. doi: 10.1128/JB.180.20.5466-5472.1998.
6
Linkage map of Escherichia coli K-12, edition 10: the traditional map.大肠杆菌K-12连锁图谱，第10版：传统图谱。
Microbiol Mol Biol Rev. 1998 Sep;62(3):814-984. doi: 10.1128/MMBR.62.3.814-984.1998.
7
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J Bacteriol. 1997 Jul;179(13):4213-8. doi: 10.1128/jb.179.13.4213-4218.1997.
8
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J Bacteriol. 1997 Jul;179(13):4206-12. doi: 10.1128/jb.179.13.4206-4212.1997.
9
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J Bacteriol. 1997 Feb;179(4):1051-8. doi: 10.1128/jb.179.4.1051-1058.1997.
10
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4
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5
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J Mol Biol. 1984 Dec 25;180(4):1023-51. doi: 10.1016/0022-2836(84)90269-9.
6
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7
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Gene. 1982 Oct;19(3):269-76. doi: 10.1016/0378-1119(82)90016-6.
9
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J Biol Chem. 1981 Nov 25;256(22):11905-10.
10
Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.利用单链噬菌体克隆辅助快速DNA测序。
J Mol Biol. 1980 Oct 25;143(2):161-78. doi: 10.1016/0022-2836(80)90196-5.